Nanoparticles (NPs) of CuBi₂O₄ with an average size around 25 nm were obtained by a novel and eco-friendly synthesis method. X-ray diffraction (XRD) and high-resolution transmission electron microscopy (HR-TEM) reveal that CuBi₂O₄ NPs possess a tetragonal crystal structure. These NPs were characterised by Raman scattering, ultraviolet-visible (UV-Vis) absorption, photoluminescence (PL), and electron paramagnetic resonance (EPR) spectroscopies. Furthermore, an in vitro study of the antibacterial effect of CuBi₂O₄ NPs against two bacterial strains, Pseudomonas aeruginosa (ATCC 43636) and Staphylococcus aureus (ATCC 25923), was carried out. The results obtained from the counting of colony-forming units (CFUs) and by the XTT-PMS cell viability assay showed an inhibition of the bacterial growth by 88%–87% for P. aeruginosa and 100% for S. aureus, at 50 mg ml⁻¹ of NPs concentration. The results of this work suggest that these nanoparticles have the potential to be used as antibacterial agents by embedding them into different surfaces or being dispersed in a coating, to prevent the bacterial colonisation.

CuBi ₂ O _{4 的}纳米颗粒（NPs）平均尺寸约为25 nm，是通过一种新型且环保的合成方法获得的。X 射线衍射 (XRD) 和高分辨率透射电子显微镜 (HR-TEM) 表明 CuBi ₂ O ₄ NPs 具有四方晶体结构。这些纳米颗粒的特征在于拉曼散射、紫外-可见光 (UV-Vis) 吸收、光致发光 (PL) 和电子顺磁共振 (EPR) 光谱。此外，CuBi ₂ O ₄ NPs 对两种细菌菌株铜绿假单胞菌(ATCC 43636) 和金黄色葡萄球菌的抗菌作用的体外研究(ATCC 25923)，进行了。从菌落形成单位 (CFU) 计数和 XTT-PMS 细胞活力测定获得的结果显示，在 50 ℃时，铜绿假单胞菌的细菌生长抑制率为 88%–87%，金黄色葡萄球菌的抑制率为100% 。 mg ml ^-1的NPs浓度。这项工作的结果表明，这些纳米颗粒有可能通过将它们嵌入不同的表面或分散在涂层中来用作抗菌剂，以防止细菌定植。