HOXA6 gene plays a role of the oncogene in various cancers. Nonetheless, its effect on gastric cancer (GC) occurrence and development is still unclear. We analysed whether HOXA6 interacts with the PBX2 protein using the STRING database. The molecular mechanism by which HOXA6 synergizes with PBX2 in GC metastasis is not fully understood. Here, we found that the expression of HOXA6 was increased in GC tissues and cell lines. The upregulation of HOXA6 was closely associated with differentiation, lymph node metastasis, AJCC stage, TNM stage, and poor survival outcome in GC patients based on tissue microarray (TMA) data. Moreover, the overexpression of HOXA6 promoted, whereas siRNA-mediated repression of HOXA6 inhibited, the cell proliferation, migration, and invasion of GC cells. Furthermore, HOXA6 could physically interact with and stabilize PBX2. In addition, HOXA6 and PBX2 expression was positively correlated in GC cells and tissue. HOXA6 and PBX2 suppression in GC cells also led to decreased migration and invasion potential in vitro. In vivo, HOXA6 was shown to cooperate with PBX2 to enhance cell metastasis via orthotopic implantation. These data indicate that HOXA6 promotes cell proliferation, migration, and invasion and that the HOXA6-PBX2 axis may be a useful biomarker for disease progression in GC.

中文翻译：

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HOXA6和PBX2的共表达促进胃癌转移

HOXA6基因在多种癌症中扮演着癌基因的角色。尽管如此，其对胃癌（GC）发生和发展的影响仍不清楚。我们使用STRING数据库分析了HOXA6是否与PBX2蛋白相互作用。尚未完全了解HOXA6与PBX2在GC转移中协同作用的分子机制。在这里，我们发现HOXA6在GC组织和细胞系中的表达增加。根据组织微阵列（TMA）数据，HOXA6的上调与分化，淋巴结转移，AJCC分期，TNM分期以及GC患者的不良生存结果密切相关。此外，HOXA6的过表达促进了，而siRNA介导的HOXA6的抑制则抑制了GC细胞的细胞增殖，迁移和侵袭。此外，HOXA6可以与PBX2物理相互作用并使其稳定。此外，HOXA6和PBX2表达在GC细胞和组织中呈正相关。GC细胞中HOXA6和PBX2的抑制也导致体外迁移和侵袭能力下降。在体内，HOXA6与PBX2协同作用，可通过原位植入增强细胞转移。这些数据表明HOXA6促进细胞增殖，迁移和侵袭，并且HOXA6-PBX2轴可能是GC中疾病进展的有用生物标记。