Biological aging is a proposed mechanism through which social determinants drive health disparities. We conducted proof-of-concept testing of eight DNA-methylation and blood-chemistry quantifications of biological aging as mediators of disparities in healthspan between Black and White participants in the United States Health and Retirement Study (HRS; n=9005). We quantified biological aging from four DNA-methylation "clocks" (Horvath, Hannum, PhenoAge, and GrimAge), a DNA-methylation Pace of Aging (DunedinPoAm), and three blood-chemistry measures (PhenoAge, Klemera-Doubal method Biological Age, and homeostatic dysregulation). We quantified Black-White disparities in healthspan from cross-sectional and longitudinal data on physical-performance tests, self-reported activities of daily living (ADL) limitations and physician-diagnosed chronic diseases, self-rated health, and survival. DNA-methylation and blood-chemistry quantifications of biological aging were moderately correlated (Pearson-r range 0.1-0.4). GrimAge, DunedinPoAm and all three blood-chemistry measures were associated with healthspan characteristics (e.g. mortality effect-size range 1.71-2.32) and showed evidence of more advanced/faster biological aging in Black compared with White participants (Cohen's d=.4-.5). These measures accounted for 13-95% of Black-White differences in healthspan-related characteristics. Findings that Black Americans are biologically older and aging more rapidly than White Americans of the same chronological age suggest that eliminating disparities in the pace of aging can contribute building to aging health equity.

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测试美国老年人生物衰老的黑白差异：DNA 甲基化和血液化学方法分析

生物老化是一种拟议的机制，社会决定因素通过该机制推动健康差异。我们对作为美国健康与退休研究 (HRS；n=9005) 中黑人和白人参与者之间健康跨度差异的中介的生物衰老的八项 DNA 甲基化和血液化学量化进行了概念验证测试。我们从四个 DNA 甲基化“时钟”（Horvath、Hannum、PhenoAge 和 GrimAge）、一个 DNA 甲基化老化速度 (DunedinPoAm) 和三个血液化学指标（PhenoAge、Klemera-Doubal 方法生物年龄、和稳态失调）。我们根据物理性能测试的横断面和纵向数据量化了健康跨度的黑白差异，自我报告的日常生活活动 (ADL) 限制和医生诊断的慢性疾病、自我评估的健康和生存。生物老化的 DNA 甲基化和血液化学定量呈中度相关（Pearson-r 范围 0.1-0.4）。GrimAge、DunedinPoAm 和所有三个血液化学指标都与健康跨度特征相关（例如死亡率效应大小范围 1.71-2.32），并且显示出与白人参与者相比，黑人更先进/更快的生物老化的证据（Cohen 的 d=.4-. 5）。这些措施占健康跨度相关特征的黑白差异的 13-95%。