Even though it is a well-accepted fact that the energy metabolism of yeast is likely to impact all cellular activities, surprising little is known about the ATP homeostasis of particular yeast strains that are commonly used in cell biological studies. Therefore, we determined key parameters such as oxygen consumption and fermentation rates of the lab strain SEY6210. Our data indicated that even at high glucose concentrations, SEY6210 produces 30-50% of cellular ATP from oxidative phosphorylation. Loss of respiration, either by disrupting ATP synthase function or by growth in anaerobic conditions, was not fully compensated by fermentation and as a result affected energy intensive processes such as the maintenance of the plasma membrane proton gradient and the associated import of nutrients.

中文翻译：

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实验室酵母菌株依靠氧化磷酸化来高效生产ATP

尽管众所周知，酵母的能量代谢可能会影响所有细胞的活动，但对于通常用于细胞生物学研究的特定酵母菌株的ATP稳态知之甚少。因此，我们确定了关键参数，例如实验室菌株SEY6210的耗氧量和发酵速率。我们的数据表明，即使在高葡萄糖浓度下，SEY6210也会通过氧化磷酸化产生30-50％的细胞ATP。通过破坏ATP合酶功能或在厌氧条件下生长导致的呼吸损失无法通过发酵得到充分补偿，因此影响了能量密集型过程，例如维持质膜质子梯度和相关的养分输入。