Abstract Introduction This scoping review explores the use of peptide microarrays in the fight against infectious diseases. The research domains explored included the use of peptide microarrays in the mapping of linear B-cell and T cell epitopes, antimicrobial peptide discovery, immunosignature characterisation and disease immunodiagnostics. This review also provides a short overview of peptide microarray synthesis. Methods Electronic databases were systematically searched to identify relevant studies. The review was conducted using the Joanna Briggs Institute methodology for scoping reviews and data charting was performed using a predefined form. The results were reported by narrative synthesis in line with the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews guidelines. Results Eighty-six articles from 100 studies were included in the final data charting process. The majority (93%) of the articles were published during 2010–2020 and were mostly from Europe (44%) and North America (34 %). The findings were from the investigation of viral (44%), bacterial (30%), parasitic (25%) and fungal (2%) infections. Out of the serological studies, IgG was the most reported antibody type followed by IgM. The largest portion of the studies (78%) were related to mapping B-cell linear epitopes, 10% were on diagnostics, 9% reported on immunosignature characterisation and 6% reported on viral and bacterial cell binding assays. Two studies reported on T-cell epitope profiling. Conclusion The most important application of peptide microarrays was found to be B-cell epitope mapping or antibody profiling to identify diagnostic and vaccine targets. Immunosignatures identified by random peptide microarrays were found to be applied in the diagnosis of infections and interrogation of vaccine responses. The analysis of the interactions of random peptide microarrays with bacterial and viral cells using binding assays enabled the identification of antimicrobial peptides. Peptide microarray arrays were also used for T-cell linear epitope mapping which may provide more information for the design of peptide-based vaccines and for the development of diagnostic reagents.

中文翻译：

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肽微阵列在对抗人类感染中的应用的范围回顾。

摘要引言本范围界定研究探讨了肽微阵列在对抗传染病中的用途。探索的研究领域包括在线性B细胞和T细胞表位的作图中使用肽微阵列，抗菌肽的发现，免疫特征鉴定和疾病免疫诊断。这篇综述还提供了肽微阵列合成的简短概述。方法系统地搜索电子数据库以识别相关研究。使用Joanna Briggs Institute方法对评论进行范围界定，并使用预定义的表格进行数据图表绘制。结果的叙述是根据叙述性综合报告进行的，符合系统评价的“首选报告项目”和“作用域评价”指南的元分析扩展。结果来自100项研究的86篇文章被纳入最终的数据制图过程。大部分文章（93％）在2010–2020年期间发表，大部分来自欧洲（44％）和北美（34％）。调查结果来自病毒（44％），细菌（30％），寄生虫（25％）和真菌（2％）感染的调查。在血清学研究中，IgG是报告最多的抗体类型，其次是IgM。研究中的大部分（78％）与B细胞线性表位的作图有关，10％与诊断有关，9％与免疫特征鉴定有关，6％与病毒和细菌细胞结合测定有关。两项研究报告了T细胞表位分析。结论发现肽微阵列最重要的应用是B细胞表位作图或抗体谱分析，以鉴定诊断和疫苗靶标。发现通过随机肽微阵列鉴定的免疫签名可用于诊断感染和询问疫苗反应。使用结合测定法分析随机肽微阵列与细菌和病毒细胞的相互作用，从而可以鉴定抗微生物肽。肽微阵列阵列也用于T细胞线性表位作图，这可能为基于肽的疫苗设计和诊断试剂的开发提供更多信息。使用结合测定法分析随机肽微阵列与细菌和病毒细胞的相互作用，从而可以鉴定抗微生物肽。肽微阵列阵列也用于T细胞线性表位作图，这可能为基于肽的疫苗设计和诊断试剂的开发提供更多信息。使用结合测定法分析随机肽微阵列与细菌和病毒细胞的相互作用，从而可以鉴定抗微生物肽。肽微阵列阵列也用于T细胞线性表位作图，这可能为基于肽的疫苗设计和诊断试剂的开发提供更多信息。