Purification of proteins from human plasma is a herculean task to perform 2-D gel electrophoresis. Human plasma contains nearly 70% albumin and globulin. The removal of such high abundance high molecular weight proteins is very difficult before performing 2-D gel electrophoresis. It becomes more difficult when we intent to investigate in infectious diseases like HIV/AIDS. We tried to the best of our efforts adopting various organic and non-organic based protocols based on various published papers. After failure of these protocols in results of 2-D gel-electrophoresis Aurum serum mini kit (Bio-Rad, USA) was adopted for plasma protein purification for performing 2-D gel electrophoresis. The low-abundance proteins were better resolved by 10% SDS-PAGE in 2-D gel-electrophoresis. Then,we extended the MALDI-TOF/TOF analysis of low-abundance proteins in human plasma by adopting the Aurum serum mini kit ( Bio-Rad, USA) for 2-D gel electrophoresis. Thus, we concluded that, depletion of high abundant proteins like albumin and globulin, the use of the Aurum serum mini kit (Bio-Rad, USA) is the protocol of choice to perform the 2-D gel electrophoresis of HIV-1 infected human plasma.

中文翻译：

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血浆蛋白纯化和2-D凝胶电泳方法对HIV-1感染的人血浆蛋白分析的比较评估

从人血浆中纯化蛋白质是执行2-D凝胶电泳的艰巨任务。人血浆中含有近70％的白蛋白和球蛋白。在进行2-D凝胶电泳之前，很难去除这种高丰度的高分子量蛋白质。当我们打算对诸如艾滋病毒/艾滋病的传染病进行调查时，这将变得更加困难。我们力求尽力采用基于各种已发表论文的各种基于有机和非有机的规程。在二维凝胶电泳结果失败后，采用Aurum血清迷你试剂盒（美国Bio-Rad）进行血浆蛋白纯化，以进行二维凝胶电泳。通过二维凝胶电泳中的10％SDS-PAGE可以更好地分离低丰度蛋白质。然后，我们通过采用Aurum血清迷你试剂盒（美国Bio-Rad）进行二维凝胶电泳，扩展了人血浆中低丰度蛋白质的MALDI-TOF / TOF分析。因此，我们得出结论，消耗高蛋白（例如白蛋白和球蛋白），使用Aurum血清迷你试剂盒（美国Bio-Rad）是进行HIV-1感染者2D凝胶电泳的选择方案等离子体。