将碱性α-羟基残基掺入原始聚酯微滴中以进行RNA分离,Biomacromolecules

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将碱性α-羟基残基掺入原始聚酯微滴中以进行RNA分离
Biomacromolecules ( IF 5.5 ) Pub Date : 2021-03-05 , DOI: 10.1021/acs.biomac.0c01697
Tony Z Jia _{1,

2} , Niraja V Bapat _{1,

3} , Ajay Verma ₃ , Irena Mamajanov ₁ , H James Cleaves _{1,

2,

4} , Kuhan Chandru _{5,

6}

Affiliation

核酸的分离和区室化可能是进化过程中原始区室系统解析的基本功能。近来，由各种α-羟基酸（αHA）的脱水合成产生的聚酯微滴被建议作为潜在的原始区室。这些液滴中的一些可以差异地分离和分隔有机染料，蛋白质和核酸。但是，先前研究的聚酯微滴包含有限的αHA化学多样性，这可能无法反映原始地球环境中可用的化学多样性。在这里，我们通过组合添加具有碱性侧链的αHA单体，4-氨基-2-羟基丁酸（4a2h）来增加聚酯微滴系统的化学多样性，将其与不同比例的其他含有不带电荷侧链的αHAs合并，通过脱水合成形成组合杂聚酯。在聚合物中掺入4a2h导致了一些能够分离荧光RNA或潜在获得固有荧光特征的聚酯微滴的组装，这表明聚酯成分的微小修饰会显着影响原始部分的功能特性。这项研究提出了一个过程，原始化学系统可以通过简单地对其化学组成进行修饰来增加其“表型”的多样性。在聚合物中掺入4a2h导致了一些能够分离荧光RNA或潜在获得固有荧光特征的聚酯微滴的组装，这表明聚酯成分的微小修饰会显着影响原始部分的功能特性。这项研究提出了一个过程，原始化学系统可以通过简单地对其化学组成进行修饰来增加其“表型”的多样性。在聚合物中掺入4a2h导致了一些能够分离荧光RNA或潜在获得固有荧光特征的聚酯微滴的组装，这表明聚酯成分的微小修饰会显着影响原始部分的功能特性。这项研究提出了一个过程，原始化学系统可以通过简单地对其化学组成进行修饰来增加其“表型”的多样性。

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Incorporation of Basic α-Hydroxy Acid Residues into Primitive Polyester Microdroplets for RNA Segregation

Nucleic acid segregation and compartmentalization were likely essential functions that primitive compartment systems resolved during evolution. Recently, polyester microdroplets generated from dehydration synthesis of various α-hydroxy acids (αHA) were suggested as potential primitive compartments. Some of these droplets can differentially segregate and compartmentalize organic dyes, proteins, and nucleic acids. However, the previously studied polyester microdroplets included limited αHA chemical diversity, which may not reflect the chemical diversity available in the primitive Earth environment. Here, we increased the chemical diversity of polyester microdroplet systems by combinatorially adding an αHA monomer with a basic side chain, 4-amino-2-hydroxybutyric acid (4a2h), which was incorporated with different ratios of other αHAs containing uncharged side chains to form combinatorial heteropolyesters via dehydration synthesis. Incorporation of 4a2h in the polymers resulted in the assembly of some polyester microdroplets able to segregate fluorescent RNA or potentially acquire intrinsic fluorescent character, suggesting that minor modifications of polyester composition can significantly impact the functional properties of primitive compartments. This study suggests one process by which primitive chemical systems can increase diversity of compartment “phenotype” through simple modifications in their chemical composition.

更新日期：2021-04-12

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