Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI,Analytical Chemistry

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Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-03-04 , DOI: 10.1021/acs.analchem.0c05277
Oliver J Hale ₁ , Helen J Cooper ₁

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Previously, we have demonstrated native mass spectrometry imaging (native MSI) in which the spatial distribution of proteins maintained in their native-like, folded conformations was determined using liquid extraction surface analysis (LESA). While providing an excellent testbed for proof of principle, the spatial resolution of LESA is currently limited for imaging primarily by the physical size of the sampling pipette tip. Here, we report the adoption of nanospray-desorption electrospray ionization (nano-DESI) for native MSI, delivering substantial improvements in resolution versus native LESA MSI. In addition, native nano-DESI may be used for location-targeted top–down proteomics analysis directly from tissue. Proteins, including a homodimeric complex not previously detected by native MSI, were identified through a combination of collisional activation, high-resolution MS and proton transfer charge reduction.

中文翻译：

纳米DESI对蛋白质和蛋白质复合物进行天然质谱成像

以前，我们已经证明了天然质谱成像（天然MSI），其中使用液体萃取表面分析（LESA）确定了保持其天然样，折叠构象的蛋白质的空间分布。尽管LESA提供了出色的测试平台以进行原理验证，但目前在成像方面，LESA的空间分辨率主要受到采样移液器吸头尖端的物理尺寸的限制。在这里，我们报道了原生MSI采用纳米喷雾解吸电喷雾电离（nano-DESI），与原生LESA MSI相比，分辨率得到了显着提高。此外，天然nano-DESI可以直接从组织中用于定位目标的自上而下的蛋白质组学分析。蛋白质，包括天然MSI以前未检测到的同型二聚体复合物，

更新日期：2021-03-16

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