The Northern house mosquito, Culex pipiens pallens, serves as important temperate vectors of several diseases, particularly the epidemic encephalitis and lymphatic filariasis. Reference genome of the Cx. pipiens pallens is helpful to understand its genomic basis underlying the complexity of mosquito biology. Using 142 Gb (∼250×) of the PacBio long reads, we assembled a draft genome of 567.56 Mb. The assembly includes 1,714 contigs with a N50 length of 0.84 Mb and a Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness of 95.6% (n = 1,367). We masked 60.63% (344.11 Mb) of the genome as repetitive elements and identified 2,032 noncoding RNAs. A total of 18,122 protein-coding genes captured a 94.1% of BUSCO gene set. Gene family evolution and function enrichment analyses revealed that significantly expanded gene families mainly involved in immunity, gustatory and olfactory chemosensation, and DNA replication/repair.

中文翻译：

---

使用 PacBio 测序的淡色库蚊（双翅目：Culicidae）基因组组装草案

北方家蚊（Culex pipiens pallens）是几种疾病的重要温带媒介，特别是流行性脑炎和淋巴丝虫病。Cx 的参考基因组。pipiens pallens 有助于了解蚊子生物学复杂性背后的基因组基础。使用 142 Gb（~250×）的 PacBio 长读长，我们组装了 567.56 Mb 的基因组草图。该组件包括 1,714 个重叠群，N50 长度为 0.84 Mb，基准通用单拷贝直系同源基因 (BUSCO) 完整性为 95.6% (n = 1,367)。我们将 60.63% (344.11 Mb) 的基因组掩盖为重复元件，并鉴定了 2,032 个非编码 RNA。共有 18,122 个蛋白质编码基因捕获了 94.1% 的 BUSCO 基因集。