A standardized data workflow is described for large-scale serum metabolomic studies using multisegment injection-capillary electrophoresis-mass spectrometry. Multiplexed separations increase throughput (<4 min/sample) for quantitative determination of 66 polar/ionic metabolites in serum filtrates consistently detected (coefficient of variance (CV) <30%) with high frequency (>75%) from a multi-ethnic cohort of pregnant women (n = 1,004). We outline a validated protocol implemented in four batches over a 7-month period that includes details on preventive maintenance, sample workup, data preprocessing and metabolite authentication. We achieve stringent quality control (QC) and robust batch correction of long-term signal drift with good mutual agreement for a wide range of metabolites, including serum glucose as compared to a clinical chemistry analyzer (mean bias = 11%, n = 668). Control charts for a recovery standard (mean CV = 12%, n = 2,412) and serum metabolites in QC samples (median CV = 13%, n = 202) demonstrate acceptable intermediate precision with a median intraclass coefficient of 0.87. We also report reference intervals for 53 serum metabolites from a diverse population of women in their second trimester of pregnancy.

描述了使用多段注射-毛细管电泳-质谱法进行大规模血清代谢组学研究的标准化数据工作流程。多重分离提高了通量（<4 分钟/样品），用于定量测定血清滤液中的 66 种极性/离子代谢物，这些代谢物在多种族队列中以高频率 (>75%) 一致检测到（方差系数 (CV) <30%）孕妇（n= 1,004）。我们概述了在 7 个月内分四批实施的经过验证的协议，其中包括有关预防性维护、样本处理、数据预处理和代谢物验证的详细信息。我们实现了严格的质量控制 (QC) 和长期信号漂移的稳健批量校正，与临床化学分析仪相比，包括血清葡萄糖在内的多种代谢物具有良好的相互一致性（平均偏差 = 11%，n = 668） . 回收标准（平均 CV = 12%，n = 2,412）和 QC 样本中的血清代谢物（中位 CV = 13%，n= 202) 表现出可接受的中间精密度，中位组内系数为 0.87。我们还报告了妊娠中期不同人群的 53 种血清代谢物的参考区间。