We inoculated Tuber aestivum and Tuber sinoaestivum on Carya illinoinensis to explore the effects of inoculation on host plant growth, enzyme activities, the physicochemical properties of rhizosphere soil, the denitrifying bacterial community in the rhizosphere, and the distribution of mating type genes in the rhizosphere. We found that the Tuber spp. inoculation increased the height of the host plant and that the stem circumference of the host was greater two months after inoculation. Six months after inoculation, the peroxidase activity of the seedlings inoculated with T. sinoaestivum was higher than that of the control. At four and six months after inoculation, the superoxidase dismutase activities of the seedlings inoculated with T. aestivum were higher than those of the seedlings inoculated with T. sinoaestivum. Six months after inoculation, nitrate nitrogen content was lowest in the control and highest in the T. sinoaestivum treatment. Among the nirS-type denitrifying bacteria community, the relative abundances of Proteobacteria were high. T. aestivum and T. sinoaestivum inoculation did not affect the diversity of denitrifying bacteria. The mating type genes MAT1-1-1 and MAT1-2-1 were detected in the rhizosphere of C. illinoinensis inoculated with T. sinoaestivum and T. aestivum, and MAT1-1-1 dominated over MAT1-2-1.

我们接种块茎小麦和块茎sinoaestivum对山核桃illinoinensis探索接种对寄主植物的生长，酶活性，根际土壤的理化性质，在根际反硝化细菌群落，以及交配型基因在根际分布的影响。我们发现块茎属。接种增加了寄主植物的高度，并且接种两个月后寄主的茎周长更大。接种 6 个月后，接种T. sinoaestivum的幼苗的过氧化物酶活性高于对照。接种后4个月和6个月，接种T. aestivum的幼苗的超氧化物歧化酶活性高于接种T. sinoaestivum的幼苗。接种后 6 个月，硝态氮含量在对照中最低，在T. sinoaestivum处理中最高。在nirS型反硝化细菌群落中，Proteobacteria 的相对丰度较高。T. aestivum和T. sinoaestivum接种不影响反硝化细菌的多样性。交配型基因MAT1-1-1和MAT1-2-1在C. illinoinensis根际被检测到接种T. sinoaestivum和T. aestivum，MAT1-1-1 优于 MAT1-2-1。