SDG711 is a histone H3K27me2/3 transmethylase in rice, a homolog of CLF in Arabidopsis, and plays key roles in regulating flowering time and panicle development. In this work, we investigated the role of SDG711 in rice seed development. Overexpression and downregulation of SDG711 lead to a decrease and increase in the expression level of genes related to starch accumulation, resulting in smaller seeds or even seed abortion. ChIP assay showed that SDG711-mediated H3K27me3 changed significantly in genes related to endosperm development, and SDG711 can directly bind to the gene body region of several starch synthesis genes and amylase genes. In addition, H3K4me3 and H3K9ac modifications also cooperate with H3K27me3 to regulate the development of the endosperm. Our results suggest that the crosstalk between SDG711-mediated H3K27me3 and H3K4me3, and H3K9ac are involved in starch accumulation to control normal seed development.

SDG711是水稻中的组蛋白H3K27me2 / 3转甲基酶，是拟南芥中CLF的同源物，在调节开花时间和穗发育中起关键作用。在这项工作中，我们调查了SDG711在水稻种子发育中的作用。SDG711的过表达和下调导致与淀粉积累相关的基因的表达水平降低和升高，从而导致种子变小甚至种子流产。ChIP分析表明，SDG711介导的H3K27me3在与胚乳发育相关的基因中发生了显着变化，并且SDG711可直接与几个淀粉合成基因和淀粉酶基因的基因体区结合。此外，H3K4me3和H3K9ac修饰也与H3K27me3协同调节胚乳的发育。我们的结果表明，SDG711介导的H3K27me3与H3K4me3和H3K9ac之间的串扰与淀粉积累有关，以控制正常种子的发育。