A multi-mycotoxin LC-MS/MS method was developed to quantify 13 free and modified Alternaria toxins in different beer types by applying a combination of stable-isotope dilution assays (SIDAs) and matrix-matched calibration. With limits of detection (LODs) between 0.03 µg/L (alternariol monomethyl ether, AME) and 5.48 µg/L (altenuene, ALT), limits of quantitation (LOQs) between 0.09 µg/L (AME) and 16.24 µg/L (ALT), and recoveries between 72 and 113%, we obtained a sensitive and reliable method, which also covers the emerging toxins alternariol-3-glucoside (AOH-3-G), alternariol-9-glucoside (AOH-9-G), alternariol monomethyl ether-3-glucoside (AME-3-G) and alternariol-3-sulfate (AOH-3-S) and alternariol monomethylether-3-sulfate (AME-3-S). Furthermore, 50 different beer samples were analyzed, showing no contamination with Alternaria toxins apart from tenuazonic acid (TeA) in concentrations between 0.69 µg/L and 16.5 µg/L. According to this study, the exposure towards TeA through beer consumption can be considered as relatively low, as the threshold of toxicological concern (TTC) value of 1500 ng/kg body weight per day might not be reached when consuming reasonable amounts of beer.

通过应用稳定同位素稀释测定 (SIDA) 和基质匹配校准相结合，开发了一种多霉菌毒素 LC-MS/MS 方法，用于定量不同类型啤酒中的 13 种游离和修饰链格孢毒素。检测限 (LOD) 介于 0.03 µg/L（交链格醇单甲醚，AME）和 5.48 µg/L（链格烯，ALT）之间，定量限 (LOQ) 介于 0.09 µg/L (AME) 和 16.24 µg/L（ ALT），回收率在 72% 至 113% 之间，我们获得了一种灵敏可靠的方法，该方法还涵盖了新出现的毒素交链格醇-3-葡萄糖苷 (AOH-3-G)、交链格醇-9-葡萄糖苷 (AOH-9-G) 、交链格醇单甲醚-3-葡萄糖苷(AME-3-G)和交链格醇-3-硫酸酯(AOH-3-S)和交链格醇单甲醚-3-硫酸酯(AME-3-S)。此外，对 50 种不同的啤酒样品进行了分析，结果显示，除了浓度在 0.69 µg/L 至 16.5 µg/L 之间的 Tenuazonic Acid (TeA) 外，没有受到链格孢毒素的污染。根据这项研究，通过饮用啤酒而接触到的TeA可以被认为是相对较低的，因为当饮用适量的啤酒时，可能无法达到每天1500纳克/公斤体重的毒理学关注（TTC）阈值。