Abstract

The HBV-related hepatocellular carcinoma (HCC) is an important liver malignancy worldwide and carries a poor prognosis. In this regard, an accurate diagnosis is necessary to enable successful treatment. The aim of the current study was to assess the relationship between the expression of certain molecular markers and HCC diagnosis in Iran. Immunohistochemistry and quantitative RT-PCR techniques were used to evaluate the expression patterns of p53 and Ki-67 in liver tissues from 121 HCC and/or HBV-infected patients and 30 healthy volunteers. Patients with HBV+HCC demonstrated increased expression of both p53 and Ki-67 compared to patients with HBV only, highlighting correlation between the p53 and Ki-67 expression levels and HCC diagnosis. The prognostic value of p53 for the diagnosis of HCC was more reliable. The p53 demonstrated higher sensitivity compared to the Ki-67 (sensitivity and specificity, 77.3 and 76.4% for the p53, and 51.0 and 97.9% for the Ki-67, respectively). A panel containing two positive markers had higher specificity and comparable sensitivity to a panel with one positive marker regardless of which one (sensitivity and specificity, 94.8 and 97.9%, for two positive markers and 96.5 and 86.4% for one positive marker, respectively). Taken together the combined analysis of p53 and Ki-67 expression provides a mean to increase the specificity and sensitivity of HBV-related HCC diagnosis to an acceptable level.

中文翻译：

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p53和Ki-67在HBV相关肝细胞癌中的表达模式：实时荧光定量PCR和免疫组织化学研究

摘要

HBV相关的肝细胞癌（HCC）是世界范围内重要的肝恶性肿瘤，预后不良。在这方面，准确的诊断对于成功治疗是必要的。本研究的目的是评估伊朗某些分子标志物的表达与HCC诊断之间的关系。免疫组织化学和定量RT-PCR技术用于评估121例HCC和/或HBV感染患者和30名健康志愿者在肝组织中p53和Ki-67的表达模式。与仅HBV患者相比，HBV + HCC患者显示p53和Ki-67的表达均增加，突显了p53和Ki-67表达水平与HCC诊断之间的相关性。p53对肝癌的预后价值更为可靠。与Ki-67相比，p53的敏感性更高（敏感性和特异性，p53的敏感性和特异性分别为77.3％和76.4％，Ki-67的敏感性和特异性分别为51.0和97.9％）。包含两种阳性标记物的面板与具有一种阳性标记物的面板相比具有更高的特异性和相当的敏感性，而与哪一种阳性标记物无关（两种阳性标记物的敏感性和特异性分别为94.8和97.9％，一种阳性标记物的敏感性和特异性分别为96.5和86.4％）。综上所述，对p53和Ki-67表达的组合分析提供了一种手段，可以将HBV相关HCC诊断的特异性和敏感性提高到可接受的水平。包含两种阳性标记物的面板与具有一种阳性标记物的面板相比具有更高的特异性和相当的敏感性，而与哪一种阳性标记物相比（对两种阳性标记物的敏感性和特异性分别为94.8和97.9％，对于一种阳性标记物的敏感性和特异性分别为96.5和86.4％）。综上所述，对p53和Ki-67表达的组合分析提供了一种手段，可以将HBV相关HCC诊断的特异性和敏感性提高到可接受的水平。包含两种阳性标记物的面板与具有一种阳性标记物的面板相比具有更高的特异性和相当的敏感性，而与哪一种阳性标记物相比（对两种阳性标记物的敏感性和特异性分别为94.8和97.9％，对于一种阳性标记物的敏感性和特异性分别为96.5和86.4％）。综上所述，对p53和Ki-67表达的组合分析提供了一种手段，可以将HBV相关HCC诊断的特异性和敏感性提高到可接受的水平。