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Macrovipera lebetina obtusa Snake Venom as a Modulator of Antitumor Effect in S-180 Sarcoma Mouse Model
Molecular Biology ( IF 1.5 ) Pub Date : 2021-03-05 , DOI: 10.1134/s0026893321020217
N. Ghazaryan , N. Movsisyan , J. C. Macedo , S. Vaz , N. Ayvazyan , L. Pardo , E. Logarinho

Abstract

Macrovipera lebetina obtusa (MLO) is a venomous snake endemic to Middle East. Here we describe the therapeutic potential of the MLO snake venom. In S-180 sarcoma-bearing mouse model, we showed that the MLO snake venom inhibits tumour growth by 50%. In human dermal microvascular endothelial cells (HMVEC-D), treatment with the MLO snake venom lead to an increase of expression levels of the vascular endothelial growth factor (VEGF), while the level of the expression of caspase 8 did not change. In HMVEC-D cells MLO snake venom induces necroptosis, rather than apoptosis. In the chick embryo chorioallantoic membrane (CAM) assay, exposure to MLO snake venom inhibited bFGF-induced angiogenesis by 22%. Taken together, these results indicate that the MLO snake venom has a potent cytotoxic activity. Regulated necroptic cell death pathway, which is engaged by MLO snake venom, may become a promising novel target for antitumor therapies.



中文翻译:

Macrovipera lebetina obtusa蛇毒作为S-180肉瘤小鼠模型中抗肿瘤作用的调节剂

摘要

大叶紫菜(MLO)是中东地区特有的毒蛇。在这里,我们描述了MLO蛇毒的治疗潜力。在具有S-180肉瘤的小鼠模型中,我们显示MLO蛇毒可抑制肿瘤生长50%。在人类真皮微血管内皮细胞(HMVEC-D)中,用MLO蛇毒进行处理可导致血管内皮生长因子(VEGF)的表达水平增加,而caspase 8的表达水平却没有变化。在HMVEC-D细胞中,MLO蛇毒会导致坏死,而不是凋亡。在鸡胚绒膜尿囊膜(CAM)分析中,暴露于MLO蛇毒可抑制bFGF诱导的血管生成22%。综上所述,这些结果表明MLO蛇毒具有有效的细胞毒活性。MLO蛇毒参与的调节性坏死细胞死亡途径,

更新日期:2021-03-05
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