For the restoration of calvarial bone defects, we have developed a biodegradable, osteogenic biocomposite material and assessed the degree of bone regeneration in rats following implantation and therapy with hyperbaric oxygen (HBO). Characterization of nano-hydroxyapatite (n-HAP) and nano-hydroxyapatite/carboxymethyl chitosan/zoledronic acid (n-HAP/CMC/ZOL) biocomposites using Fourier-transform infrared spectroscopy (FTIR) showed the existence of the apatite phosphate group and the spectra of X-ray diffraction (XRD) displayed a polycrystalline existence. The morphological characterization of n-HAP and biocomposite using scanning electron microscopy (SEM) showed the porous spherical shape, and the inclusion of calcium and phosphate as the major elements was shown by the energy dispersive X-ray (EDX) spectrum. Using osteoblast MG-63 cells, the n-HAP/CMC/ZOL biocomposite was evaluated to show effective cytocompatibility. To assess potential effects of hyperbaric oxygen (HBO) on biocomposite substitutes were applied to standard bone defects in rat tibiae. The findings indicate that, contrasted with the monitor, there was substantially more new bone forming in the HBO group. The use of this novel material with HBO treatment may offer an alternative to autogenous bone grafting.

为了恢复颅骨缺损，我们开发了一种可生物降解的成骨生物复合材料，并评估了高压氧（HBO）植入和治疗后大鼠的骨再生程度。使用傅立叶变换红外光谱（FTIR）对纳米羟基磷灰石（n-HAP）和纳米羟基磷灰石/羧甲基壳聚糖/唑来膦酸（n-HAP / CMC / ZOL）生物复合材料进行表征，结果表明存在磷灰石磷酸盐基团，并且光谱X射线衍射（XRD）图像显示了多晶的存在。使用扫描电子显微镜（SEM）对n-HAP和生物复合材料进行形态学表征显示出多孔球形形状，并且通过能量色散X射线（EDX）光谱显示了以钙和磷酸盐为主要元素的夹杂物。使用成骨细胞MG-63细胞，对n-HAP / CMC / ZOL生物复合材料进行了评估，以显示有效的细胞相容性。为了评估高压氧（HBO）对生物复合材料替代物的潜在影响，将其应用于大鼠胫骨的标准骨缺损。研究结果表明，与监测者相比，HBO组中明显有更多的新骨形成。将此新材料与HBO处理结合使用可为自体骨移植提供替代方法。