To encapsulate piperine (Pip), as a poor water-soluble bioactive compound, zein-sodium caseinate-xanthan gum (Z-SG-XG) nanocomplex was prepared as a colloidal delivery system. The effect of different parameters involved in complexation process, including concentration of proteins, polysaccharide, and Pip on the encapsulation efficiency of Pip, particle size and stability of the nanocomplexes was investigated. Powders obtained by freeze-drying of the colloidal solution had relatively uniform particles compared to those obtained from conventional drying system and showed well redispersibility in water. At the optimal condition, a stable and homogeneous nanocomplex with a mean particle size of 145.9 ± 2.7 nm, PDI of 0.27 ± 0.01, and ζ-potential of −39.7 ± 1.3 mV was obtained. The antioxidant activity of Pip was significantly improved by encapsulation into the Z-SC-XG nanocomplex. Also, the in vitro release of Pip from the synthesized nanocomplexes in phosphate-buffer saline (PBS) solution and simulated gastrointestinal fluids (SGIF) was investigated and the release kinetic was studied as well. The Pip/Z-SG-XG nanocomplex showed a slower release in SGIF compared to the free Pip and nanoparticles without XG and SC, while its antioxidant activity was remarkable. Results suggested a possible utilization of Z-SC-XG nanocomplex for improving the water solubility, bioavailability and storage stability of Pip.

为了封装胡椒碱（Pip），作为水溶性差的生物活性化合物，制备了玉米醇溶蛋白-酪蛋白酸钠-黄原胶（Z-SG-XG）纳米复合物作为胶体递送系统。研究了复合过程中涉及的不同参数（包括蛋白质，多糖和Pip的浓度）对Pip包封效率，粒径和纳米复合物稳定性的影响。与从常规干燥系统获得的粉末相比，通过胶体溶液的冷冻干燥获得的粉末具有相对均匀的颗粒，并且在水中显示出良好的再分散性。在最佳条件下，获得了稳定均匀的纳米复合物，其平均粒径为145.9±2.7 nm，PDI为0.27±0.01，ζ电位为-39.7±1.3 mV。通过封装到Z-SC-XG纳米复合物中，Pip的抗氧化活性得到了显着改善。另外，还研究了在磷酸盐缓冲盐水（PBS）和模拟胃肠液（SGIF）中从合成的纳米复合物中Pip的体外释放，并研究了释放动力学。与不含XG和SC的游离Pip和纳米颗粒相比，Pip / Z-SG-XG纳米复合物在SGIF中的释放较慢，而其抗氧化活性却非常出色。结果表明，Z-SC-XG纳米复合物可用于改善Pip的水溶性，生物利用度和储存稳定性。研究了磷酸盐缓冲盐水（PBS）和模拟胃肠液（SGIF）中合成纳米复合物对Pip的体外释放，并研究了其释放动力学。与不含XG和SC的游离Pip和纳米颗粒相比，Pip / Z-SG-XG纳米复合物在SGIF中的释放较慢，而其抗氧化活性却非常出色。结果表明，Z-SC-XG纳米复合物可用于改善Pip的水溶性，生物利用度和储存稳定性。研究了磷酸盐缓冲盐水（PBS）和模拟胃肠液（SGIF）中合成纳米复合物对Pip的体外释放，并研究了其释放动力学。与不含XG和SC的游离Pip和纳米颗粒相比，Pip / Z-SG-XG纳米复合物在SGIF中的释放较慢，而其抗氧化活性却非常出色。结果表明，Z-SC-XG纳米复合物可用于改善Pip的水溶性，生物利用度和储存稳定性。