Abstract

Upconversion nanoparticles (UCNPs) are a promising nanoplatform for bioreagent formation for in vivo imaging, which emit UV and blue light under the action of near-infrared radiation, providing deep tissue penetration and maintaining a high signal-to-noise ratio. In the case of solid tumor visualization, the UCNP surface functionalization is required to ensure a long circulation time, biocompatibility, and non-toxicity. The effective UCNP accumulation in the solid tumors is determined by the disturbed architecture of the vascular network and lymphatic drainage. This work demonstrates an approach to the UCNP biofunctionalization with endogenous polysialic acid for in vivo bioreagent formation. Bioreagents possess a low level of nonspecific protein adsorption and macrophage uptake, which allow the prolongation of the circulation time in the bloodstream up to 3 h. This leads to an intense photoluminescent signal in the tumor.

中文翻译：

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用聚唾液酸修饰的上转换纳米颗粒用于体内实体肿瘤的可视化

摘要

上转换纳米粒子（UCNPs）是一种很有前途的用于体内成像生物试剂形成的纳米平台，它在近红外辐射的作用下发出紫外线和蓝光，提供深层组织穿透并保持高信噪比。在实体瘤可视化的情况下，需要 UCNP 表面功能化以确保长循环时间、生物相容性和无毒。实体瘤中有效的 UCNP 积累取决于血管网络和淋巴引流的结构紊乱。这项工作展示了一种用内源性聚唾液酸进行 UCNP 生物功能化的方法，用于体内生物试剂的形成。生物试剂具有低水平的非特异性蛋白质吸附和巨噬细胞摄取，这允许将血液循环时间延长至 3 小时。这导致肿瘤中强烈的光致发光信号。