Royal jelly is one of the most remarkable commercial bee products used in cosmetics, medicine, and as a dietary supplement. This study aimed to determine some quality parameters (amino acid, multi-elemental, total phenolic-flavonoid, 10-HDA, sugar, and moisture content) as well as antioxidant and antimicrobial activities of different royal jelly samples (domestic and non-domestic samples). In this study, royal jelly samples exhibited high antioxidant and antimicrobial activity. 10-HDA values were found to be higher in domestic royal jelly samples (2.17–2.71%) compared to non-domestic samples (0.31–1.71%). Also, it was found that the acidity value varied between 2.57–3.37 mL 0.1 N NaOH/g and 3.87–4.97 mL 0.1 N NaOH/g in non-domestic and domestic royal jelly samples, respectively. L-Proline was the amino acid with the highest rate of occurrence in both domestic (21972.39–35053.00 nmol/g) and non-domestic (19688.31–21711.50 nmol/g) samples. It was determined that the new method developed for amino acid analysis has many advantages over other methods. Chemometrics of principal component analysis (PCA) showed that amino acid profile, elemental profile, and chemical parameters were capable of presenting characteristic and descriptive properties of royal jelly samples. In addition to these findings, Raman spectroscopy combined with chemometrics of hierarchical cluster analysis (HCA) was successfully used to distinguish domestic and non-domestic royal jelly samples. The obtained results will provide an important basis for subsequent research on the evaluation of the amino acid content, physicochemical properties, phenolic content, and the antioxidant and elemental profile of royal jelly.

蜂王浆是最出色的商业蜂产品之一，用于化妆品、医药和膳食补充剂。本研究旨在确定不同蜂王浆样品（国产和非国产样品）的一些质量参数（氨基酸、多元素、总酚类黄酮、10-HDA、糖和水分含量）以及抗氧化和抗菌活性。 ）。在这项研究中，蜂王浆样品表现出很高的抗氧化和抗菌活性。与非国内样品 (0.31-1.71%) 相比，国内蜂王浆样品 (2.17-2.71%) 的 10-HDA 值更高。此外，还发现非国产和国产蜂王浆样品的酸度值分别在 2.57-3.37 mL 0.1 N NaOH/g 和 3.87-4.97 mL 0.1 N NaOH/g 之间变化。L-脯氨酸是国内（21972.39-35053.00 nmol/g）和非国内（19688.31-21711.50 nmol/g）样品中出现率最高的氨基酸。确定为氨基酸分析开发的新方法比其他方法具有许多优点。主成分分析 (PCA) 的化学计量学表明，氨基酸谱、元素谱和化学参数能够呈现蜂王浆样品的特征和描述特性。除了这些发现之外，拉曼光谱结合层次聚类分析 (HCA) 的化学计量学也成功地用于区分国产和非国产蜂王浆样品。所得结果将为后续氨基酸含量、理化性质的评价研究提供重要依据。