A small-scale survey was conducted on 12 beehives located in the Gharb region of Morocco in order to assess for the first time the presence of honey bee-infecting viruses in the country. A total of 240 individual bee samples were screened for seven honey bee viruses using reverse transcription (RT)-PCR. Among the infected samples, 41.7% contained one virus, 15% showed mixed infection with two viruses while 3.3% contained three. Deformed wing virus (DWV), was the most prevalent one, detected in 38.3% of the samples, followed by acute bee paralysis virus (ABPV), sacbrood virus (SBV) and chronic bee paralysis virus (CBPV) (20%, 14.2% and 9.2% of infection rate, respectively). Israeli acute paralysis virus (IAPV), Kashmir bee virus (KBV) and black queen cell virus (BQCV) were not detected. Nucleotide sequences of PCR amplicons obtained from detected viruses shared 98.5-99.4% identity with isolates reported in the GenBank. Although bee viruses usually persist as asymptomatic infections and persist in the colonies as covert infections, they can dramatically affect honey bee health and shorten the lives of infected bees under certain conditions. To the best of our knowledge, this study represents the first detection of ABPV, CBPV and SBV in honey bees in Morocco.

对位于摩洛哥 Gharb 地区的 12 个蜂箱进行了小规模调查，以首次评估该国是否存在感染蜜蜂的病毒。使用逆转录 (RT)-PCR 对总共 240 个蜜蜂样本进行了七种蜜蜂病毒的筛选。在被感染的样本中，41.7%含有一种病毒，15%表现为两种病毒混合感染，3.3%含有三种病毒。变形翼病毒 (DWV) 是最普遍的病毒，在 38.3% 的样本中检测到，其次是急性蜜蜂麻痹病毒 (ABPV)、囊虫病毒 (SBV) 和慢性蜜蜂麻痹病毒 (CBPV)（20%、14.2%）和 9.2% 的感染率）。未检测到以色列急性麻痹病毒（IAPV）、克什米尔蜜蜂病毒（KBV）和黑蜂王细胞病毒（BQCV）。从检测到的病毒中获得的 PCR 扩增子的核苷酸序列与 GenBank 中报告的分离株具有 98.5-99.4% 的同一性。尽管蜜蜂病毒通常以无症状感染的形式持续存在，并以隐性感染的形式持续存在于蜂群中，但它们会极大地影响蜜​​蜂的健康，并在某些条件下缩短受感染蜜蜂的寿命。据我们所知，这项研究代表了摩洛哥蜜蜂中 ABPV、CBPV 和 SBV 的首次检测。