Abstract

Purpose

To evaluate the antitumor efficacy in local and distant tumors induced by local hyperthermia with CTLA-4 blockade

Methods

A mouse breast cancer cell line was inoculated into both sides of the legs of mice. The mice were treated with three administrations of CTLA-4 blockade, a single application of local hyperthermia (42.5 °C for 20 min) to the tumor on one side of the leg, or the combination of the two. Tumor growth in locally heated tumors (HT tumors) and unheated distant tumors (UnHT tumors) and overall survival were evaluated.

Results

In the combination group, tumor volume significantly decreased for both HT and UnHT tumors compared with the tumors in the untreated and local hyperthermia monotherapy groups. Remarkable efficacy was only observed in the combination therapy group, in which 7 of 18 mice responded to HT and UnHT tumors, with significant prolonged overall survival.

Conclusions

Combination therapy enhanced the antitumor response not only in HT tumors but also in UnHT tumors and prolonged overall survival.

中文翻译：

---

局部热疗结合CTLA-4阻断在小鼠乳腺癌模型中既诱导局部又引起抽象性效应

摘要

目的

评估CTLA-4阻断剂对局部高温引起的局部和远处肿瘤的抗肿瘤作用

方法

将小鼠乳腺癌细胞系接种到小鼠腿的两侧。小鼠接受了三次CTLA-4阻断治疗，对腿部一侧肿瘤局部应用局部热疗（42.5°C，持续20分钟）或两者结合治疗。评估了局部加热的肿瘤（HT肿瘤）和未加热的远处肿瘤（UnHT肿瘤）中的肿瘤生长以及总体生存率。

结果

在组合组中，与未经治疗的局部热疗和局部热疗单药治疗组相比，HT和UnHT肿瘤的肿瘤体积均显着减少。仅在联合治疗组中观察到了显着的疗效，其中18只小鼠中有7只对HT和UnHT肿瘤有反应，总体生存期显着延长。

结论

联合治疗不仅在HT肿瘤中而且在UnHT肿瘤中均增强了抗肿瘤反应，并延长了总生存期。