Capsicum chinense Jacq. is classified under the Solanaceae family, which is an extensively consumed spice and vegetable globally. Therefore, to gain more knowledge and insight into the diversity of Capsicum chinense Jacq. metabolites, a total of 18 placental tissues from various development stages were collected and untargeted metabolomics was conducted by means of ultra-performance liquid chromatography (UPLC) and mass spectrometry (MS). Principal component analysis (PCA) analysis established the existence of distinct metabolite distribution patterns as observed at 16 days post anthesis (DPA), compared with the metabolites at 36 and 48 DPA groups, whereas there was a difference in metabolites between the orange ripening period (B) and the red ripening period (C), which intersected with each other. Furthermore, several pathways including metabolic pathways, biosynthesis of phenylpropanoids, ABC transporters, alanine, aspartate and glutamate metabolism, fatty acid biosynthesis, pentose and glucoronate pathways, secondary metabolites biosynthesis, cutin, biosynthesis of suberine and wax were significantly enriched across the fruit ripening stages. The capsaicin content was observed to be less in the early ripening stages, but gradually increased to a high concentration during the late ripening stages. In conclusion, our study findings submit a suitable approach for interpreting the biochemical variances of non-targeted metabolomics in hot pepper developmental stages, as well as offer new findings that can be applied in the development strategies in breeding of Capsicum chinense Jacq.

中文翻译：

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通过辣椒的代谢组学分析生化差异。在水果发育过程中

黄灯笼辣椒黄灯笼。被归类为茄科（Solanaceae family），茄科是全球范围内广泛食用的香料和蔬菜。因此，要获得更多关于辣椒的多样性的知识和见识。雅克 代谢物，总共收集了来自不同发育阶段的18个胎盘组织，并通过超高效液相色谱（UPLC）和质谱（MS）进行了非靶向代谢组学。与36和48个DPA组的代谢产物相比，主成分分析（PCA）分析建立了在花后16天（DPA）观察到的独特代谢产物分布模式，而橙色成熟期之间的代谢产物存在差异（ B）和红色成熟期（C）相互交叉。此外，还有几种途径，包括代谢途径，苯丙烷类化合物的生物合成，ABC转运蛋白，丙氨酸，天冬氨酸和谷氨酸代谢，脂肪酸生物合成，戊糖和葡萄糖酸途径，次级代谢产物生物合成，角质，在整个果实成熟阶段，地下和蜡的生物合成显着丰富。辣椒素的含量在成熟初期较少，但在成熟后期逐渐增加至高浓度。总之，我们的研究结果为解释辣椒发育阶段非靶向代谢组学的生化差异提供了一种合适的方法，并提供了可用于玉米育种发展策略的新发现。黄灯笼辣椒黄灯笼。