Previous genetic pest management (GPM) systems in diamondback moth (DBM) have relied on expressing lethal proteins (‘effectors’) that are ‘cell-autonomous’, that is, they do not leave the cell in which they are expressed. To increase the flexibility of future GPM systems in DBM, we aimed to assess the use of a non-cell-autonomous, invertebrate-specific, neurotoxic effector – the scorpion toxin AaHIT. This AaHIT effector was designed to be secreted by expressing cells, potentially leading to effects on distant cells, specifically neuromuscular junctions.

中文翻译：

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无脊椎动物特异性蝎子毒素 AaHIT 的工程表达降低了小菜蛾 Plutella xylostella 的成虫寿命和雌性繁殖力

以前的小菜蛾 (DBM) 遗传害虫管理 (GPM) 系统依赖于表达“细胞自主”的致死蛋白（“效应子”），也就是说，它们不会离开表达它们的细胞。为了提高未来 GPM 系统在 DBM 中的灵活性，我们旨在评估非细胞自主、无脊椎动物特异性、神经毒性效应物——蝎子毒素 AaHIT 的使用。这种 AaHIT 效应器被设计为通过表达细胞分泌，可能导致对远处细胞的影响，特别是神经肌肉接头。