The novel interaction mode among centromere sub-complex CENP-O/P/U/Q/R,Journal of Molecular Recognition

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The novel interaction mode among centromere sub-complex CENP-O/P/U/Q/R
Journal of Molecular Recognition ( IF 2.3 ) Pub Date : 2021-03-03 , DOI: 10.1002/jmr.2892
Beibei Cao ₁ , Congcong Zhao ₁ , Yu Zhang ₁ , Xiaoyu Wang ₁ , Jingjing Ye ₁ , Liqiao Hu ₁ , Xiaojing He ₁

Affiliation

The kinetochore is essential for the accurate segregation of sister chromosome in the eukaryote cell. Among the kinetochore subunits, five proteins CENP-O/P/U/Q/R form a stable complex, referred to as CENP-O class, and are required for proper kinetochore function. Although the function and structure of yeast COMA complex (CENP-O/P/U/Q homologs) have been revealed extensively, the assembly mechanism and detail interactions among human CENP-O class are significantly different and remain largely unclear. Here, we identified the fragment (residues 241-360) of CENP-U and the C-terminal half of CENP-Q are essential to form a hetero-complex and interact with CENP-O/P sub-complex in vitro. We for the first time showed that CENP-R does not directly interact with CENP-O/P in vitro, but indeed interact with CENP-U and CENP-Q. Furthermore, both the N- and C-terminus of CENP-R are required for the interaction with CENP-U and CENP-Q. Our research pinpointed a novel interaction pattern that might shed light on the assembly mechanism of vertebrate CENP-O class.

中文翻译：

着丝粒亚复合体 CENP-O/P/U/Q/R 之间的新型相互作用模式

动粒对于真核细胞中姐妹染色体的准确分离至关重要。在着丝粒亚基中，五种蛋白质 CENP-O/P/U/Q/R 形成一个稳定的复合物，称为 CENP-O 类，是适当的着丝粒功能所必需的。尽管酵母 COMA 复合物（CENP-O/P/U/Q 同源物）的功能和结构已被广泛揭示，但人类 CENP-O 类之间的组装机制和详细相互作用却存在显着差异，并且在很大程度上仍不清楚。在这里，我们确定了 CENP-U 的片段（残基 241-360）和 CENP-Q 的 C 端半部分对于在体外形成异质复合物并与 CENP-O/P 亚复合物相互作用是必不可少的。我们首次证明 CENP-R 在体外不直接与 CENP-O/P 相互作用，但确实与 CENP-U 和 CENP-Q 相互作用。此外，CENP-R 的 N 端和 C 端都是与 CENP-U 和 CENP-Q 相互作用所必需的。我们的研究确定了一种新的相互作用模式，可能有助于阐明脊椎动物 CENP-O 类的组装机制。

更新日期：2021-03-03

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