Plants, through the photosynthesis process, produce the substances necessary for all the life cycles of nature, which are called "primary metabolites." Moreover, there are some plants that synthesize, in addition to these, other substances with more specific functions, which are known as "secondary metabolites." It is inside this group that flavonoids are located, whose main function is to protect organisms from damage caused by different oxidizing agents. Luteolin (3,4,5,7‐tetrahydroxy‐flavone) belongs to the sub‐class of flavonoids known as flavones and is one of 10,000 flavonoids currently known, being one of the most bio‐active flavonoids. Its various beneficial properties for health, together with the increasing reduction in the use of synthetic antioxidants, make the study of luteolin a very active field. Within this, the quantification of this molecule has become a subject of very special interest given that it is transversal to all fields. In this review article, we aim to give the reader a broad and deep vision of this topic, focusing on the events reported in the last 5 years and covering all possible techniques related to analytical determinations. We will discuss in terms of advantages and disadvantages between techniques, selectivity, sensitivity, costs, time consumption, and reagents as well as in the complexity of operations.

中文翻译：

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木犀草素的分析测定

植物通过光合作用过程，产生自然界所有生命周期所必需的物质，称为“初级代谢产物”。而且，除了这些之外，还有一些植物会合成其他具有更特殊功能的物质，这些物质被称为“次级代谢产物”。黄酮类化合物位于该组内，其主要功能是保护生物体免受不同氧化剂的伤害。木犀草素（3,4,5,7-四羟基黄酮）属于黄酮类黄酮的一个亚类，是目前已知的 10,000 种黄酮类化合物之一，是最具生物活性的黄酮类化合物之一。它对健康的各种有益特性，加上合成抗氧化剂的使用越来越少，使木犀草素的研究成为一个非常活跃的领域。这其中，鉴于该分子跨越所有领域，因此对其进行量化已成为一个非常令人感兴趣的主题。在这篇评论文章中，我们旨在让读者对该主题有一个广泛而深入的了解，重点关注过去 5 年报告的事件并涵盖与分析测定相关的所有可能技术。我们将讨论技术、选择性、灵敏度、成本、时间消耗和试剂之间的优缺点以及操作的复杂性。重点关注过去 5 年报告的事件并涵盖与分析测定相关的所有可能技术。我们将讨论技术、选择性、灵敏度、成本、时间消耗和试剂之间的优缺点以及操作的复杂性。重点关注过去 5 年报告的事件并涵盖与分析测定相关的所有可能技术。我们将讨论技术、选择性、灵敏度、成本、时间消耗和试剂之间的优缺点以及操作的复杂性。