The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), is considered as one of the most important pests of potato plants. Control of CPB is mainly based on chemical insecticides. However, control failure is often reported because of the rapid resistance development ability of CPB; therefore, there is an urgent need for integrated control methods. In this study, we evaluated the combined usage of insecticides chlorantraniliprole, spinosad, and spinetoram, which are registered for CPB control, with the entomopathogenic nematode Steinernema feltiae (Rhabditida: Steinernematidae).

In addition, the efficacy of S. feltiae on a chlorantraniliprole-resistant CPB population exposed to synergistic chemicals PBO (Piperonyl butoxide), DEM (Diethyl maleate) and DEF (S,S,S-tributylphorotrithioate) inhibiting certain detoxification enzyme groups was determined. The tested insecticides showed no adverse effect on the survival and infectivity of S. feltiae and the isolate was considered compatible with integrated CPB control. This study also showed that pre-treatment with synergistic chemicals (particularly PBO and DEM) increased the efficacy of S. feltiae against CPB. To our knowledge, this is the first study determining the usage of entomopathogenic nematode + synergist combination. Therefore, further studies on the potential use of synergistic chemicals in combination with entomopathogenic nematodes should be performed under both laboratory and field conditions.

科罗拉多马铃薯甲虫（CPB），马铃薯Leptinotarsa decemlineata（Say）（鞘翅目：金眼科）被认为是马铃薯植物中最重要的害虫之一。CPB的控制主要基于化学杀虫剂。然而，由于CPB的快速电阻形成能力，经常会报告控制失败。因此，迫切需要集成控制方法。在这项研究中，我们评估了已注册用于CPB防治的杀虫剂绿藻腈，多杀菌素和多杀菌素与昆虫病原线虫Steinernema feeliae（Rhabditida：Steinernematidae）的组合使用。

另外，测定了链霉菌对暴露于协同化学药品PBO（戊基丁醇），DEM（马来酸二乙酯）和DEF（S，S，S-三丁基三氟甲基硫代乙酸酯）抑制某些排毒酶基团的耐氯吡喹的CPB种群的功效。所测试的杀虫剂对毡状链球菌的存活和传染性没有不利影响，该分离物被认为与综合CPB控制兼容。这项研究还表明，用协同化学物质（尤其是PBO和DEM）进行预处理可以提高苦参的功效。反对CPB。就我们所知，这是确定昆虫病原线虫+增效剂组合用法的第一项研究。因此，应在实验室和野外条件下对协同化学与线虫病线虫结合使用的潜在用途进行进一步的研究。