The frequent application of β cypermethrin in farming activity, causing severe soil and water contamination. Thus, finding a suitable microbial agent to degrade the toxic pesticide into less or nontoxic components is vital. Hence, β cypermethrin-resistant predominant bacteria from the pesticide-exposed surface of cotton leaves were isolated and optimized the growth conditions required for the significant degradation of β cypermethrin. Six dominant bacterial cultures were isolated from pesticide exposed cotton leaf samples, and among them, COL3 showed better tolerance to 6% of β cypermethrin than others. This COL3 was identified as Streptomyces toxytricini D2 through the 16S rRNA analysis. The suitable growth requirements of S. toxytricini D2 were optimized with various essential growth parameters to degrade β cypermethrin and the results showed that a significant degradation of β cypermethrin was observed at 35 °C, pH 8.0, 1.5% of inoculum, and nutritional factors like glycerol (20 mg L⁻¹), ammonium sulfate (15 mg L⁻¹), and calcium phosphates (10 mg L⁻¹) were served as better carbon, nitrogen, and phosphate sources respectively. The degradation percentage and half-life of β cypermethrin were calculated as 80.71 ± 1.17% and 48.15 h respectively by S. toxytricini D2. The GC-MS analysis results showed that S. toxytricini D2 effectively degraded the β cypermethrin into 5 components such as methyl salicylate, phenol, phthalic acid, 3-phenoxy benzaldehyde, and 3-PBA. This is the first report, revealed that the S. toxytricini D2 belongs to the Actinobacteria has the potential to degrade the β cypermethrin into less or nontoxic metabolites under optimized conditions.

β氯氰菊酯在耕作活动中的频繁应用，导致严重的土壤和水污染。因此，找到合适的微生物剂以将有毒农药降解成较少或无毒的成分至关重要。因此，从暴露于农药的棉叶表面分离出了对β氯氰菊酯具有抗性的优势细菌，并优化了有效降解β氯氰菊酯所需的生长条件。从接触农药的棉叶样品中分离出六种优势细菌培养物，其中COL3对β氯氰菊酯的耐受性优于其他细菌。通过16S rRNA分析，该COL3被鉴定为毒链霉菌D2。毒链霉菌的适宜生长要求用各种必需的生长参数对D2进行了优化，以降解β-氯氰菊酯，结果表明，在35°C，pH 8.0，接种物的1.5％和营养因子（如甘油，20 mg L ^-1）下，β-氯氰菊酯的降解显着。分别以硫酸铵（15 mg L ^-1）和磷酸钙（10 mg L ^-1）作为更好的碳源，氮源和磷酸盐源。β氯氰菊酯的降解率和半衰期分别通过计算为80.71±1.17％和48.15ħ S. toxytricini D2。GC-MS分析结果表明，链霉菌D2有效地将β氯氰菊酯降解为5种成分，例如水杨酸甲酯，苯酚，邻苯二甲酸，3-苯氧基苯甲醛和3-PBA。这是第一份报告，揭示了属于放线菌的毒素链球菌D2具有在最佳条件下将β氯氰菊酯降解为较少或无毒代谢产物的潜力。