This work aims to investigate the inhibitory effect of crotonaldehyde manufacture wastewater (CMW) on biological acidification. To reveal the inhibitory effect of wastewater to the anaerobic granular sludge (AnGS), variations of the specific acidogenic activity (SAA) and activities of key enzymes were investigated. The results indicated that the dosage of CMW causing a 50% effect concentration (EC₅₀) on the activity of total volatile fatty acids (TVFA) production was 380 mg COD/g VSS. The inhibitory effect of individual toxicants in CMW on the activity of TVFA production were in the order of crotonaldehyde·ethyl sorbate·(E,E)-2,4-hexadienal, and their inhibitory degrees on individual VFA productions were acetic acid (Ac)·n-butyric acid (n-Bu), which could correspond to the variations in the activities of acetate kinase (AK) and butyrate kinase (BK). Furthermore, the combined effect of three toxicants on the activity of TVFA production was significantly higher than that of any individual toxicant, and the contribution of the relative toxicity to CMW was 77.27%. Additionally, the biodegradation products of the main toxicants indicated that the removal ofcrotonaldehyde and (E,E)-2,4-hexadienal was primarily due to the hydrogenation of alkene and aldehyde and the oxidation of aldehyde. Nevertheless, the removal of ethyl sorbate was primarily based on adsorption. In conclusion, biological acidification has a limited ability to treatment CMW, therefore, a further pretreatment technology should be used to remove the main toxicant of wastewater.

这项工作旨在调查巴豆醛生产废水（CMW）对生物酸化的抑制作用。为了揭示废水对厌氧颗粒污泥（AnGS）的抑制作用，研究了比生酸活性（SAA）和关键酶活性的变化。结果表明CMW的剂量引起50％的有效浓度（EC ₅₀）对总挥发性脂肪酸（TVFA）产生的活性为380 mg COD / g VSS。CMW中有毒物质对TVFA产生活性的抑制作用依次为巴豆醛·山梨酸乙酯·（E，E）-2,4-己二醛，其对VFA产生的抑制程度为乙酸（Ac）。 ·正丁酸（n-Bu），可能对应于乙酸激酶（AK）和丁酸激酶（BK）活性的变化。此外，三种毒物对TVFA产生活性的综合影响明显高于任何一种单独的毒物，相对毒性对CMW的贡献为77.27％。此外，主要毒物的生物降解产物表明，巴豆醛和（E，E）-2，4-己二醛主要是由于烯烃和醛的氢化以及醛的氧化。然而，山梨酸乙酯的去除主要基于吸附。总之，生物酸化处理CMW的能力有限，因此，应使用进一步的预处理技术去除废水中的主要有毒物质。