The exponential rise in the Nigerian population has necessitated the use of agrochemicals for enhanced agricultural yields to meet the ever-rising demand for food. However, agrochemicals such as organochlorine pesticides (OCPs) have caused several devastating health and ecological challenges. The study was therefore aimed at assessing the bioaccumulation of OCPs and the associated parasitological and microbial susceptibility in P. obscura to determine the possible ecological impacts of the chemical. A total of 106 specimens of Parachanna obscura fish species were sampled between July and November 2019 from Lekki Lagoon in Lagos, Nigeria. Four culture media, namely nutrient agar (NA), MacConkay agar (MCA), eosin methylene blue (EMB), and sabouraud dextrose agar (SDA) were employed in microbial culture. These microbes were subjected to ceftazidime, ceftriaxone, cefuroxime, gentamicin, ofloxacin, augmentin, nitrofurantoin, ciprofloxacin, and erythromycin to test for resistance, susceptibility and intermediate statuses before and after curing. OCPs were tested in the water, sediment, and tissues of P. obscura using gas chromatography flame ionization detector (GC-FID). P. obscura sampled in the lagoon had poor growth exponent which was characterized by negative allometry (slenderness) in the sampled fish. Although the incidence of parasitic infection in the fish was not alarming, the situation might be aggravated if the prevalent anthropogenic activities persist, resulting in immunosuppression. Regulation of anthropogenic activities in the catchment area is recommended to forestall the prognosis of health and environmental hazards associated with the agricultural, industrial, pharmaceutical, and municipal activities around the lagoon. Bacteria that conferred the most resistance to the majority of the antibiotics were Staphylococcus sp., Micrococcus sp. Escherichia coli and Klebsiella sp., testing positive to plasmid profile. They conferred high resistance to the antibiotics before plasmid curing but became highly susceptible post- plasmid curing. This implies that the gene for resistance in the bacteria isolates was plasmid-mediated, that is, they were obtained from the environment. In the event of an outbreak of waterborne diseases such as cholera, typhoid, dysentery, and diarrhea, there may be non-response to treatment among the infected inhabitants. The incidence of antibiotic resistance in bacteria colonies recorded in this study is of great public health concern, given the possibility of the antibiotic-resistant bacteria strains being passed to humans through fish consumption, resulting in increased multi-drug resistance in humans. Regulation of anthropogenic activities around the lagoon is recommended to forestall prognosis of health and environmental hazards associated with OCPs from agricultural, industrial, pharmaceutical, and municipal sources.

中文翻译：

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生物积累的有机氯农药，Procamallanus sp。（Baylis，1923年）从尼日利亚拉各斯Lekki泻湖采样的非洲蛇头鱼中的感染和微生物定植

尼日利亚人口的指数增长使必须使用农药来提高农业产量，以满足不断增长的食品需求。但是，诸如有机氯农药（OCP）之类的农用化学品已经造成了几项破坏性的健康和生态挑战。因此，该研究旨在评估隐孢假单胞菌中OCP的生物蓄积性以及相关的寄生虫学和微生物敏感性，以确定该化学药品可能产生的生态影响。2019年7月至11月之间，从尼日利亚拉各斯的莱基泻湖采样了总共106种Parachanna obscura鱼物种标本。在微生物培养中使用了四种培养基，即营养琼脂（NA），麦康凯琼脂（MCA），曙红亚甲基蓝（EMB）和沙堡葡萄糖葡萄糖琼脂（SDA）。这些微生物受到头孢他啶的影响，头孢曲松，头孢呋辛，庆大霉素，氧氟沙星，增强素，硝基呋喃妥因，环丙沙星和红霉素在固化前后测试其耐药性，药敏性和中间状态。使用气相色谱火焰电离检测器（GC-FID）在暗黑假单胞菌的水，沉积物和组织中测试了OCP。在泻湖中取样的暗黑假单胞菌的生长指数较差，其特征是在取样的鱼中呈异形（细长）。尽管鱼中寄生虫感染的发生率并不令人震惊，但如果普遍的人为活动持续存在，可能会加剧这种情况，从而导致免疫抑制。建议规范集水区的人为活动，以预防与农业，工业，制药，环礁湖周围的市政活动。对大多数抗生素产生最大抵抗力的细菌是葡萄球菌，微球菌。大肠杆菌和克雷伯菌属，对质粒图谱呈阳性。它们在质粒固化前赋予了对抗生素的高抗性，但在质粒固化后变得高度敏感。这意味着细菌分离物中的抗性基因是质粒介导的，也就是说，它们是从环境中获得的。如果发生霍乱，伤寒，痢疾和腹泻等水传播疾病，感染的居民可能对治疗无反应。这项研究中记录的细菌菌落中的抗生素耐药性发生率是引起公众极大关注的问题，考虑到食用抗生素的细菌可能会通过食用鱼类传给人类，从而导致人类对多种药物的耐药性增加。建议规避泻湖周围的人为活动，以预防与农业，工业，制药和市政来源的OCP相关的健康和环境危害的预后。