Regarding the proven anticonvulsant effect of Zhumeria majdae essential oil (ZMEO) in previous studies we were prompted to investigate the ZMEO effects on the tolerance to the anticonvulsant effects of morphine and the morphine withdrawal syndrome. Tolerance to the morphine anticonvulsant effect was induced in mice by subcutaneous injection of 2.5 mg/kg of morphine for 4 days. Subsequent doses of ZMEO (20 mg/kg) were used to study the expression and development of morphine tolerance. Clonidine was used as the standard drug to inhibit the morphine withdrawal syndrome symptoms. To study the ZMEO effect on withdrawal syndrome, mice received appropriate morphine values for 4 days and on the fifth day, 60 min before administration of naloxone. The effective dose of ZMEO was determined and the number of jumps, stands and changes in the dry stool weight, as symptoms of withdrawal syndrome were evaluated. The dose of 20 mg/kg of ZMEO decreased the tolerance in development and expression groups significantly. Counting the number of jumping, standing and defecation were assessed 30 min after morphine and 1 h after the vehicle and clonidine. The dose of 40 mg/kg ZMEO decreased all the signs of withdrawal syndrome significantly. ZMEO was analyzed by GC/MS and linalool (53.1%) and camphor (23.8%) were characterized as the main components. The results suggest that ZMEO possesses constituent(s) that have activity against tolerance to the anticonvulsant effects of morphine and the morphine withdrawal symptoms.

中文翻译：

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玛氏假单胞菌精油的化学成分及其对吗啡戒断综合征的表达及对吗啡的抗惊厥作用的耐受性，对戊四氮诱发的小鼠癫痫发作

关于先前的研究中证明的马氏假单胞菌精油（ZMEO）的抗惊厥作用，我们被提示研究ZMEO对吗啡和吗啡戒断综合征的抗惊厥作用的耐受性。通过皮下注射2.5 mg / kg吗啡4天，诱导了小鼠对吗啡抗惊厥作用的耐受性。随后剂量的ZMEO（20 mg / kg）用于研究吗啡耐受性的表达和发展。使用可乐定作为抑制吗啡戒断综合征症状的标准药物。为了研究ZMEO对戒断综合征的作用，在给予纳洛酮之前的4天和第5天（60分钟），小鼠接受了适当的吗啡值。确定ZMEO的有效剂量，并确定跳跃次数，站立次数和干粪重量的变化，评估戒断综合症的症状。20 mg / kg的ZMEO剂量显着降低了发育和表达组的耐受性。吗啡注射后30分钟，溶媒和可乐定注射1小时后，对跳跃，站立和排便的次数进行计数。40 mg / kg ZMEO剂量可显着降低戒断综合症的所有症状。ZMEO进行了GC / MS分析，以芳樟醇（53.1％）和樟脑（23.8％）为主要成分。结果表明，ZMEO具有对吗啡的抗惊厥作用和吗啡戒断症状具有耐受性的活性成分。吗啡后30分钟，溶媒和可乐定1小时后评估站立和排便情况。40 mg / kg ZMEO剂量可显着降低戒断综合症的所有症状。ZMEO进行了GC / MS分析，以芳樟醇（53.1％）和樟脑（23.8％）为主要成分。结果表明，ZMEO具有对吗啡的抗惊厥作用和吗啡戒断症状具有耐受性的活性成分。吗啡后30分钟，溶媒和可乐定1小时后评估站立和排便情况。40 mg / kg ZMEO的剂量可显着降低戒断综合症的所有症状。ZMEO进行了GC / MS分析，以芳樟醇（53.1％）和樟脑（23.8％）为主要成分。结果表明，ZMEO具有对吗啡的抗惊厥作用和吗啡戒断症状具有耐受性的活性成分。