One aquatic coleopteran species from family Dytiscidae and two aquatic coleopteran genera from family Hydrophilidae were recorded in the summer period and represent first records in the Egyptian lakes. Beetles were collected from two northern lakes, Lake Idku and Lake Burullus. They were identified by morphological characteristics as well as the mtDNA barcoding method. A molecular phylogenetic approach was used to determine the genetic identity of the collected samples based on the mitochondrial cytochrome oxidase I (COI). Prodaticus servillianus (Dytiscidae) from Egypt showed no significant difference in the COI region and they are highly similar to P. servillianus from Madagascar. The phylogenetic analysis revealed that the other two coleopteran genera belong to family Hydrophilidae. Based on COI only, there is no clear evidence for their genetic identity at the species level. So, we defined them to the closest taxon and denoted them as Cymbiodyta type A and B. The results indicated that resolving the molecular identity of the aquatic beetles from northern lakes of Egypt need more considerations in the field of biological conservation. We concluded that utilization of COI as a barcoding region for identifying some coleopteran species is not sufficient and additional molecular markers are required to uncover the molecular taxonomy at deep levels.

中文翻译：

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DNA条形码揭示了埃及北部湖泊中三个罕见鞘翅目属的最早记录。

夏季记录了一种来自Dytiscidae科的水生鞘翅目物种和两个亲水科的水生鞘翅目属，这是埃及湖泊中的第一个记录。甲虫从两个北部湖泊Idku湖和Burullus湖采集。通过形态特征以及mtDNA条形码方法对它们进行了鉴定。基于线粒体细胞色素氧化酶I（COI），使用了一种分子系统发育方法来确定所收集样品的遗传同一性。埃及的Prodaticus servillianus（Dytiscidae）在COI地区没有显着差异，并且与马达加斯加的P. servillianus非常相似。系统发育分析表明，另外两个鞘翅目属属于亲水科。仅基于COI，没有明确证据表明它们在物种一级具有遗传身份。因此，我们将它们定义为最接近的分类单元，并将其命名为Cymbiodyta类型A和B。结果表明，在生物保护领域，解析埃及北部湖泊水生甲虫的分子身份需要更多考虑。我们得出的结论是，利用COI作为识别某些鞘翅目物种的条形码区域是不够的，还需要其他分子标记来揭示深层次的分子分类学。