Species of Trichogramma Westwood, 1833 (Hymenoptera: Trichogrammtidae) are frequently used as biological control agents against Lepidoptera, but practical application of these egg endoparasitoids are complicated because of their complex taxonomy. This study aimed to compare sequences of internal transcribed spacer regions of ribosomal DNA (ITS2-rDNA) of Trichogramma accessions with those deposited in GenBank in order to access the reliability of the ITS2 as a barcode for discriminating species and evaluating the genetic diversity. ITS2-rDNA sequences obtained from seventeen specimens of Trichogramma confirmed previous identifications based on morphological characteristics. Multiple sequence alignment revealed the existence of highly conserved regions in ITS2 sequences while the neighbour-joining dendrogram indicated that the specimens formed three clusters comprising T. manicobai and T. marandobai (group I), T. galloi (group II) and T. pretiosum (group III). The ITS2 marker was shown to be a powerful DNA barcode for discriminating Trichogramma species and could be used to complement the morphological approach.

中文翻译：

---

rDNA内部转录间隔区2（ITS2）区域的序列分析，用于鉴定赤眼蜂属物种和评估遗传多样性

Trichogramma Westwood，1833年的种（膜翅目：Trichogrammtidae）经常用作鳞翅目的生物防治剂，但由于它们的生物分类复杂，这些卵类内寄生虫的实际应用非常复杂。这项研究的目的是比较赤眼蜂属种的核糖体DNA（ITS2-rDNA）的内部转录间隔区序列与GenBank中保存的序列，以获取ITS2作为区分物种和评估遗传多样性的条形码的可靠性。从十七个赤眼蜂标本中获得的ITS2-rDNA序列证实了先前基于形态学特征的鉴定。多重序列比对揭示了ITS2序列中高度保守的区域的存在，而相邻的树状图表明，标本形成了三个簇，包括马尼伯白蚁和马氏白蚁（I组），鸡毛白蚁（II组）和百日草（T. pretiosum） （第三组）。ITS2标记被证明是一种强大的DNA条形码，可用于区分赤眼蜂属物种，可用于补充形态学方法。