Toward High-Throughput Fish Embryo Toxicity Tests in Aquatic Toxicology,Environmental Science & Technology

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Toward High-Throughput Fish Embryo Toxicity Tests in Aquatic Toxicology
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2021-03-03 , DOI: 10.1021/acs.est.0c07688
Donald Wlodkowic ₁ , Olivia Campana ₂

Affiliation

Addressing the shift from classical animal testing to high-throughput in vitro and/or simplified in vivo proxy models has been defined as one of the upcoming challenges in aquatic toxicology. In this regard, the fish embryo toxicity test (FET) has gained significant popularity and wide standardization as one of the sensitive alternative approaches to acute fish toxicity tests in chemical risk assessment and water quality evaluation. Nevertheless, despite the growing regulatory acceptance, the actual manipulation, dispensing, and analysis of living fish embryos remains very labor intensive. Moreover, the FET is commonly performed in plastic multiwell plates under static or semistatic conditions, potentially inadequate for toxicity assessment of some organic, easily degradable or highly adsorptive toxicants. Recent technological advances in the field of mechatronics, fluidics and digital vision systems demonstrate promising future opportunities for automation of many analytical stages in embryo toxicity testing. In this review, we highlight emerging advances in fluidic and laboratory automation systems that can prospectively enable high-throughput FET testing (HT-FET) akin to pipelines commonly found in in vitro drug discovery pipelines. We also outline the existing challenges, barriers to future development and provide an outlook of ground-breaking fluidic technologies in embryo toxicity testing.

中文翻译：

对水生毒理学中高通量鱼胚胎毒性测试的支持

解决从经典动物测试到体外高通量和/或简化的体内代理模型的转变已被定义为水生毒理学中即将面临的挑战之一。在这方面，作为化学风险评估和水质评估中急性鱼毒性测试的敏感替代方法之一，鱼胚胎毒性测试（FET）已获得广泛普及和广泛标准化。然而，尽管越来越多的法规接受，但是活鱼胚胎的实际操作，分配和分析仍然是劳动密集型的。此外，场效应管通常在静态或半静态条件下在塑料多孔板中进行，可能不足以评估某些有机，易降解或高吸附性的有毒物质的毒性。机电一体化，流体技术和数字视觉系统领域的最新技术进展表明，在胚胎毒性测试中许多分析阶段实现自动化的前景广阔。在本文中，我们重点介绍了流体和实验室自动化系统中的新兴进展，这些进展有望有望实现类似于体外药物发现管道中常见管道的高通量FET测试（HT-FET）。我们还将概述现有挑战，未来发展的障碍，并提供胚胎毒性测试中突破性的流体技术的前景。我们重点介绍了流体和实验室自动化系统中的新兴进展，这些进展有望使高通量FET测试（HT-FET）类似于体外药物发现管道中常见的管道。我们还将概述现有挑战，未来发展的障碍，并提供胚胎毒性测试中突破性的流体技术的前景。我们重点介绍了流体和实验室自动化系统中的新兴进展，这些进展有望使高通量FET测试（HT-FET）类似于体外药物发现管道中常见的管道。我们还将概述现有的挑战，未来发展的障碍，并提供胚胎毒性测试中突破性的流体技术的前景。

更新日期：2021-03-16

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