We conducted a study to assess the accuracy of nearest neighbour (NN) and multiple hypothesis tracking (MHT) methods—which are opposite extremes in computational complexity—in determining the percentage of motile sperms and the number of sperms tracked in simulated data of fish sperm movements, and to evaluate the resulting number of tracking errors and analysis duration. Sperm tracking and swimming path assembly were assessed in 36 video clips (1 s length at 100 fps) of emulated Rhamdia quelen sperm kinetics at different densities (50, 100, 200 or 300 spermatozoa in the field of view) and motility rates (30, 60 or 90%). The MHT method accurately estimated the percentage of motile sperms, whereas NN underestimated it by up to 6.59%. Increase in sperm density reduced the number of sperms tracked from both trackers. With more than 50 sperms in the field of view, NN and MHT tracked 73% and 92% of the ground-truth sperm count, respectively. Both trackers showed a quadratic increase in tracking errors with increasing sperm density. The maximum percentage of errors at 90% motility was 12% for NN and 4.7% for MHT. The MHT tracker required up to 150 s to track 300 sperms, whereas NN completed the tracking procedure in less than 0.5 s. On maintaining a density of up to 100 sperms in the field of view, it was possible to obtain high accuracy, low number of tracking errors and an acceptable analysis duration with both tracking methods.

中文翻译：

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比较从跟踪方法中的两个计算极端获得的鱼精子活力测量的准确性：最近邻和多假设跟踪

我们进行了一项研究，以评估最近邻 (NN) 和多假设跟踪 (MHT) 方法（这两种方法在计算复杂性方面是相反的极端）在确定鱼精子模拟数据中活动精子的百分比和跟踪的精子数量方面的准确性运动，并评估由此产生的跟踪错误数量和分析持续时间。在模拟的Rhamdia quelen 的36 个视频剪辑（1 s 长度，100 fps）中评估了精子跟踪和游泳路径组装不同密度（视野中有 50、100、200 或 300 个精子）和运动率（30、60 或 90%）下的精子动力学。MHT 方法准确估计了活动精子的百分比，而 NN 低估了它高达 6.59%。精子密度的增加减少了两个追踪器追踪的精子数量。由于视野中有 50 多个精子，NN 和 MHT 分别跟踪了真实精子数量的 73% 和 92%。两个追踪器都显示出随着精子密度的增加，追踪误差的二次增加。NN 在 90% 运动时的最大错误百分比为 12%，MHT 为 4.7%。MHT 跟踪器需要长达 150 秒来跟踪 300 个精子，而 NN 在不到 0.5 秒的时间内完成了跟踪过程。在视野中保持高达 100 个精子的密度时，可以获得高精度，