The aim of the study was to determine the ruminal degradability of dry matter (DM), daidzein, genistein, glycitein and total isoflavones in ground full-fat soybean (GFFS) and solvent-extracted soybean meal (SSBM) using the in sacco method. The experiment was carried out in three replications on ruminally cannulated sheep that were fed twice a day with a diet consisted of hay and supplemental mixture (6:4, DM basis). The nylon bags with 2 g feed samples ground to 2 mm were incubated in the rumen for 0, 2, 4, 8, 16 and 24 h. The effective degradability (ED) of DM, daidzein, genistein, glycitein and total isoflavones was calculated at outflow rate of 0.06 h. The ED of DM in GFFS was 77.8% and was higher than in SSBM being 71.8% (p < 0.001). The ED of daidzein (96.8%) and genistein (93.6%) was higher for SSBM compared with GFFS (93.9% and 92.8%, p < 0.001 and p = 0.003, respectively) while ED of glycitein was lower for SSBM than for GFFS (75.5 and 81.7%, respectively, p < 0.001). All isoflavones in the incubations were extensively degraded in the rumen, and regardless of dietary source, they were almost completely degraded after 16 h of incubation. Further, the disappearance patterns, that is the functions describing the time courses of the analyte disappearance, were assessed. The disappearance patterns of daidzein, genistein, glycitein and total isoflavones were similar and showed greater disappearance of mentioned isoflavones from SSBM compared to GFFS (p < 0.001 for daidzein, genistein and total isoflavones and p = 0.002 for glycitein). The study provides knowledge on the effect of processing on degradability of isoflavones in rumen that can be used to clarify the interrelationship between isoflavones and rumen microbiota.

中文翻译：

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全脂大豆和豆粕中异黄酮的瘤胃降解性评价——初步研究


本研究的目的是使用in sacco法测定全脂大豆粉 (GFFS) 和溶剂提取豆粕 (SSBM) 中干物质 (DM)、大豆苷元、染料木黄酮、黄豆黄素和总异黄酮的瘤胃降解性。该实验在瘤胃插管羊上进行了三次重复，每天喂食两次由干草和补充混合物（6:4，DM 基础）组成的饮食。将装有研磨至2mm的2g饲料样品的尼龙袋在瘤胃中孵育0、2、4、8、16和24小时。在0.06 h的流出速率下计算DM、大豆苷元、染料木黄酮、黄豆黄素和总异黄酮的有效降解率(ED)。 GFFS 中 DM 的 ED 为 77.8%，高于 SSBM 中的 71.8% ( p < 0.001)。与 GFFS 相比，SSBM 中黄豆苷元 (96.8%) 和染料木黄酮 (93.6%) 的 ED 较高（分别为 93.9% 和 92.8%， p < 0.001 和p = 0.003），而 SSBM 中黄豆黄素的 ED 低于 GFFS（分别为 75.5 和 81.7%， p < 0.001）。孵化中的所有异黄酮在瘤胃中都被广泛降解，并且无论饮食来源如何，在孵化16小时后它们几乎完全降解。此外，还评估了消失模式，即描述分析物消失时间进程的函数。大豆黄酮、染料木黄酮、黄豆黄素和总异黄酮的消失模式相似，并且与 GFFS 相比，SSBM 中提及的异黄酮消失程度更大（大豆黄酮、染料木黄酮和总异黄酮的p < 0.001，黄豆黄素的p = 0.002）。 该研究提供了有关加工对瘤胃中异黄酮降解性影响的知识，可用于阐明异黄酮与瘤胃微生物群之间的相互关系。