Identification and characterization of sex‐biased and differentially expressed miRNAs in gonadal developments of the Chinese mitten crab, Eriocheir sinensis,Molecular Reproduction and Development

当前位置： X-MOL 学术 › Mol. Reprod. Dev. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Identification and characterization of sex‐biased and differentially expressed miRNAs in gonadal developments of the Chinese mitten crab, Eriocheir sinensis
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2021-03-03 , DOI: 10.1002/mrd.23459
Bi-Yun Luo ₁ , Xin-Yi Xiong ₁ , Xue Liu ₁ , Xue-Ying He ₁ , Gao-Feng Qiu ₁

Affiliation

MicroRNA (miRNA) is a posttranscriptional downregulator that plays a vital role in a wide variety of biological processes. In this study, we constructed five ovarian and testicular small RNA libraries using two somatic libraries as reference controls for the identification of sex‐biased miRNAs and gonadal differentially expressed miRNAs (DEMs) of the Chinese mitten crab, Eriocheir sinensis. A total of 535 known and 243 novel miRNAs were identified, including 312 sex‐biased miRNAs and 402 gonadal DEMs. KEGG pathway analysis showed that DEM target genes were statistically enriched in MAPK, Wnt, and GnRH signaling pathway, and so on. A number of the sex‐biased miRNAs target genes associated with sex determination/differentiation, such as IAG, Dsx, Dmrt1, and Fem1, while others target the genes related to gonadal development, such as P450s, Wnt, Ef1, and Tra‐2c. Dual‐luciferase reporter assay in vitro further confirmed that miR‐34 and let‐7b can downregulate IAG expression, miR‐9‐5p, let‐7d, let‐7b, and miR‐8915 can downregulate Dsx. Taken together, these data strongly suggest a potential role for the sex‐biased miRNAs in sex determination/differentiation and gonadal development in the crab.

更新日期：2021-03-25

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11