A multi-barrier antibiotics loaded biodegradable composite bone cement for resolving chronic osteomyelitis has been studied to understand the physico-mechanical properties, drug loading/eluting efficiency, and different merits and demerits prior to clinical application. After successful induction of bone infection in 28 rabbits using methicillin-resistant Staphylococcus aureus (MRSA) strains, calcium sulfate/bioactive glass based composite cement was implanted in 12 defects to assess its performance over parenteral therapy with microscopic and radiological examination for 90 days. The composite cement revealed acceptable physico-mechanical properties and controlled drug elution kinetics. Furthermore, the antibiotics concentrations in bone up to 42 days were sufficient to kill MRSA without eliciting adverse drug reactions. The striking feature of platelets aggregation by composite cement could assist bone healing. The controlled degradation with simultaneous entrapment of composite cement within the osteoid tissues and complete repair of infected cortical defects (holes) in rabbit tibia at 6 weeks indicated the excellent anti-infective and osteoconductive properties of composite cement. Thus, the animal study demonstrated the superiority of composite over injectable antibiotic therapy based on infection resolution and bone regeneration. We thereby conclude that the composite cement can be effectively applied in the treatment of resistant cases of chronic osteomyelitis.

已经研究了一种用于解决慢性骨髓炎的多屏障抗生素负载可生物降解复合骨水泥，以了解临床应用前的物理力学特性、载药/洗脱效率以及不同的优缺点。使用耐甲氧西林金黄色葡萄球菌成功诱导 28 只兔子的骨感染后(MRSA) 菌株，硫酸钙/生物活性玻璃基复合水泥被植入 12 个缺损处，以通过显微镜和放射检查 90 天评估其在肠外治疗中的性能。复合水泥显示出可接受的物理机械性能和受控的药物洗脱动力学。此外，长达 42 天的骨中抗生素浓度足以杀死 MRSA 而不会引起药物不良反应。复合水泥对血小板聚集的显着特征有助于骨愈合。在 6 周时，复合水泥在类骨质组织内的受控降解和完全修复兔胫骨中感染的皮质缺损（孔）表明复合水泥具有优异的抗感染和骨传导性能。因此，动物研究证明了基于感染解决和骨再生的复合疗法优于注射抗生素疗法。因此，我们得出结论，复合水泥可以有效地用于治疗慢性骨髓炎的耐药病例。