This study aimed to investigate the effect of swine manure composting with microbial inoculation (MI) and without MI (CK) on heavy metal (Cr, Cd, and Pb) fractions, humic substance (HS), and metabolism pathway. The results showed that MI could passivate the heavy metal Cr and reduce the proportion of exchangeable (EXC) fraction of Cd, but it does not affect the EXC fraction of Pb. Compared to CK, HS, humic acid (HA), and fulvic acid (FA) were significantly increased with MI at the maturity stage. The propagation of Proteobacteria (day 4) and Firmicutes (days 12 and 24) was strengthened with MI. Canonical correlation analysis found that HA and Firmicutes were positively correlated with heavy metal (Cr, Cd, and Pb) residual (RES) fraction, and FA was positively correlated with Proteobacteria. Moreover, MI can significantly increase amino acid metabolism and carbohydrate metabolism by day 4, enhance the metabolism of enzyme families and glycan biosynthesis by day 12, and improve membrane transport. Overall, MI could facilitates the increase in HA and FA content and transfer of heavy metal (Cr, Cd, and Pb) fractions, it particularly helps increase the RES fraction.

这项研究的目的是调查猪粪堆肥微生物接种（MI）和无微生物接种（CK）对重金属（Cr，Cd和Pb）级分，腐殖质（HS）和代谢途径的影响。结果表明，MI可以钝化重金属Cr，降低Cd中可交换（EXC）部分的比例，但不影响Pb的EXC部分。与CK相比，MI在成熟阶段显着增加HS，腐殖酸（HA）和黄腐酸（FA）。心肌梗死加强了Proteobacteria（第4天）和Fimicutes（第12和24天）的繁殖。典型相关分析发现，HA和Firmicutes与重金属（Cr，Cd和Pb）残留（RES）分数呈正相关，而FA与变形杆菌呈正相关。而且，MI可以在第4天显着增加氨基酸代谢和碳水化合物代谢，在第12天可以增强酶家族的代谢和聚糖的生物合成，并改善膜运输。总体而言，MI可以促进HA和FA含量的增加以及重金属（Cr，Cd和Pb）馏分的转移，特别是有助于提高RES分数。