The Mediterranean fruit fly (medfly), Ceratitis capitata, is a worldwide pest of agriculture able to use olfactory cues to locate habitat, food sources, mates and oviposition sites. The sensitivity of medfly olfaction has been exploited to develop olfactory-based attractants that are currently important tools for detection, control and eradication of its populations. Among these is Cera Trap^® (BIOIBERICA, S.A.U.), a cost-effective bait. Here we used coupled gas chromatography/electroantennographic detection (GC-EAD) and GC/mass spectrometry (GC-MS) approaches to characterize the medfly antennally-active compounds released by this lure. We identified GC peaks corresponding to chemicals belonging to six different classes including heterocyclic aromatic compounds, aliphatic alcohols, aldehydes, esters, sesquiterpene hydrocarbons, and aromatic alcohols. We tested ten potential candidate volatiles belonging to these classes and predicted to be emitted by the lure and found that they were eliciting electroantennographic responses in medfly adults. These results will help in unravelling the physiological mechanisms of odor perception in both sexes, especially in relation to Cera Trap^® attractant activity, which in the field has been shown to be female-specific. These findings and their developments will ultimately expand the toolbox for medfly control in the field.

地中海果蝇（地中海实蝇），地中海实蝇，是农业能够利用嗅觉线索找到栖息地，食物来源，队友和产卵站点的全球害虫。medfly嗅觉的敏感性已被用来开发基于嗅觉的引诱剂，这些引诱剂目前是检测，控制和消除其种群的重要工具。其中之一是剑桥能源研究协会陷阱^®（BIOIBERICA，SAU），一种经济有效的诱饵。在这里，我们使用了气相色谱/电造影检测（GC-EAD）和气相色谱/质谱（GC-MS）的方法来表征由这种诱饵释放的蝇蝇触媒活性化合物。我们确定了与六种不同类别的化学物质相对应的GC峰，这些化学物质包括杂环芳族化合物，脂族醇，醛，酯，倍半萜碳氢化合物和芳族醇。我们测试了属于这些类别的十种潜在候选挥发物，并预测它们会被诱剂释放，并发现它们正在引起蝇蝇成虫的电子触角反应。这些结果将有助于解开气味感知的生理机制，在男女双方，尤其是有关Cera的陷阱^®诱剂活性，在该领域已被证明是女性特有的。这些发现及其发展最终将扩展该领域的medfly控制工具箱。