Gentiana kurroo Royle is a critically endangered medicinal herb of the Indian Himalaya. It has bioactive seco-iridoid glycosides, notably gentiopicroside, in the rhizome and roots. In this study, we report on the in vitro production of gentiopicroside (GPD) from adventitious (AD) roots induced directly from the leaf, nodal, and suspension cell cultures of G. kurroo. Murashige and Skoog (MS) media fortified with Indole-3-butyric acid (IBA) 2 mg L⁻¹ + Naphthaleneacetic acid (NAA) 0.5 mg L⁻¹ and IBA 2 mg L⁻¹ + Indole-3-acetic acid (IAA) 1 mg L⁻¹ produced the maximum number of roots. Suspension cell cultures derived AD roots showed a 1.41-fold higher biomass production than adventitious roots induced from leaf and nodal explants. Among the various concentrations of MS media salts evaluated, half-strength MS suspension media favored the higher biomass and GPD accumulation. The maximum accumulation of GPD (2.58 mg g ^–l dry weight (DW)) and AD root biomass (18.96 g L⁻¹ DW) was observed on the 48^th day. Furthermore, GPD produced from AD root cultures were separated by using TLC, characterized using Nuclear Magnetic Resonance (NMR) and High-Resolution Liquid Chromatograph-Mass Spectrometer (HR LC-MS) and quantified with High-Performance Liquid Chromatography (HPLC).

Gentiana kurroo Royle是印度喜马拉雅山的极度濒危草药。它在根茎和根中具有生物活性的山梨糖苷糖苷，尤其是龙胆苦苷。在这项研究中，我们报告了从G. kurroo的叶片，淋巴结和悬浮细胞培养物中直接诱导的不定根（AD）根的龙胆苦苷（GPD）的体外生产。Murashige和Skoog（MS）介质用吲哚-3-丁酸（IBA）2 mg L ^-1 +萘乙酸（NAA）0.5 mg L ^-1和IBA 2 mg L ^-1 +吲哚-3-乙酸（IAA ）强化）1毫克L ^-1产生最大数量的根。悬浮细胞培养物衍生的AD根显示出的生物量比叶片和节点外植体诱导的不定根高1.41倍。在所评估的各种浓度的MS培养基盐中，半强度MS悬浮培养基偏向于更高的生物量和GPD积累。GPD的最大累积（2.58毫克克^-l干重（DW））和AD根生物量（18.96克大号^-1对48中观察到DW）^第一天。此外，使用TLC分离从AD根培养物中产生的GPD，使用核磁共振（NMR）和高分辨率液相色谱-质谱仪（HR LC-MS）进行表征，并通过高效液相色谱（HPLC）进行定量。