A four-year survey (2012–2015) was carried out to examine the genetic diversity of cucumber mosaic virus (CMV) isolates infecting tomato, as well as the presence and diversity of their satellite RNAs (satRNAs), collecting a total of 226 samples throughout the most important growing regions in Serbia. Besides CMV-like symptoms, the collected samples also exhibited more severe symptoms, such as systemic necrosis of leaves, branches and stems, accompanied by fruit malformation and necrosis. In a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), the presence of CMV was detected in approximately one quarter of the tested samples. Identification of CMV was confirmed by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of the coat protein (CP) gene of a group of 11 selected Serbian CMV isolates. Phylogenetic analysis of the CMV CP sequences of these selected isolates revealed their heterogeneity, as they fell into two different subgroups, IA and II. An additional RT-PCR analysis of CMV positive isolates using satellite RNAs specific primers detected the presence of satRNAs in eight samples. Sequence and phylogenetic analyses showed that Serbian CMV satRNAs variants were very heterogeneous, belonging to necrogenic and non-necrogenic variants. Necrogenic variants were divided into two groups, B and B1, containing a characteristic ‘necrogenic consensus’ sequence at the 3′ end of the RNA. A necrotic phenotype co-determined by satRNAs was expressed in inoculated tomato plants.

进行了为期四年的调查（2012-2015年），以检查感染番茄的黄瓜花叶病毒（CMV）分离株的遗传多样性以及其卫星RNA（satRNA）的存在和多样性，共收集了226个样本塞尔维亚最重要的增长地区。除了CMV样症状外，采集的样品还表现出更严重的症状，例如叶片，树枝和茎的系统性坏死，并伴有水果畸形和坏死。在双抗体夹心酶联免疫吸附测定（DAS-ELISA）中，在大约四分之一的测试样品中检测到CMV的存在。通过常规逆转录聚合酶链反应（RT-PCR）和一组11个选定的塞尔维亚CMV分离株的外壳蛋白（CP）基因测序，证实了CMV的鉴定。对这些分离株的CMV CP序列进行系统进化分析，揭示了它们的异质性，因为它们分为两个不同的亚组IA和II。使用卫星RNA特异性引物对CMV阳性分离株进行的另一种RT-PCR分析检测到八个样品中存在satRNA。序列和系统发育分析表明，塞尔维亚CMV satRNAs变异非常异质，属于致死性和非致死性变异。致死性变体分为B和B1两组，在RNA的3'端含有一个特征性的“致死性共有”序列。由satRNA共同确定的坏死表型在接种的番茄植株中表达。使用卫星RNA特异性引物对CMV阳性分离株进行的另一种RT-PCR分析检测到八个样品中存在satRNA。序列和系统发育分析表明，塞尔维亚CMV satRNAs变异非常异质，属于致死性和非致死性变异。致死性变体分为B和B1两组，在RNA的3'端含有一个特征性的“致死性共有”序列。由satRNA共同确定的坏死表型在接种的番茄植株中表达。使用卫星RNA特异性引物对CMV阳性分离株进行的另一种RT-PCR分析检测到八个样品中存在satRNA。序列和系统发育分析表明，塞尔维亚CMV satRNAs变异非常异质，属于致死性和非致死性变异。致死性变体分为B和B1两组，在RNA的3'端含有一个特征性的“致死性共有”序列。由satRNA共同确定的坏死表型在接种的番茄植株中表达。致死性变体分为B和B1两组，在RNA的3'端含有一个特征性的“致死性共有”序列。由satRNA共同确定的坏死表型在接种的番茄植株中表达。致死性变体分为B和B1两组，在RNA的3'端含有一个特征性的“致死性共有”序列。由satRNA共同确定的坏死表型在接种的番茄植株中表达。