Fluorescent proteins (FPs) are reported to play an important role as photoprotectants and antioxidants in corals subjected to stressful conditions. Identifying the various FP genes expressed and FP gene expression patterns under stress in diverse coral species can provide insight into FP function. In this study, we identified 16 putative FP homologues from the transcriptomes of corals with varying susceptibility to elevated temperature, including Acropora digitifera, Favites colemani, Montipora digitata and Seriatopora caliendrum. Each coral expressed a different complement of FP transcripts, which were predicted to have distinct spectral properties. The most diverse and abundant repertoire of FP transcripts, including at least 6 green FPs, were expressed in the temperature-tolerant coral, F. colemani. In comparison, the other corals expressed fewer FP types. Specific FP transcripts exhibited variable expression profiles in coral fragments subjected to 32 ± 1 °C (treatment) or 28 ± 1 °C (control) for up to 72 h, suggesting that distinct FPs may have different roles. Further studies on the expression of the proteins encoded by these FP transcripts, their fluorescence activity, tissue localization, and possible antioxidant properties, are needed to reveal their contribution to thermal stress tolerance in certain species of corals.

中文翻译：

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耐温和易感造礁珊瑚中荧光蛋白的表达

据报道，荧光蛋白 (FPs) 在遭受压力条件的珊瑚中作为光保护剂和抗氧化剂发挥重要作用。识别不同珊瑚物种在压力下表达的各种 FP 基因和 FP 基因表达模式可以提供对 FP 功能的深入了解。在这项研究中，我们从珊瑚转录组中鉴定了 16 个推定的 FP 同源物，它们对升高的温度具有不同的敏感性，包括鹿角珊瑚,最爱科勒曼尼,指状蒙蒂波拉和轮虫. 每个珊瑚都表达了不同的 FP 转录本，预计它们具有不同的光谱特性。最多样化和最丰富的 FP 转录本，包括至少 6 个绿色 FP，在耐温珊瑚中表达，F.科勒曼尼. 相比之下，其他珊瑚表达的 FP 类型较少。特定的 FP 转录本在 32 ± 1 °C（处理）或 28 ± 1 °C（对照）长达 72 小时的珊瑚片段中表现出可变的表达谱，这表明不同的 FP 可能具有不同的作用。需要进一步研究这些 FP 转录本编码的蛋白质的表达、它们的荧光活性、组织定位和可能的抗氧化特性，以揭示它们对某些珊瑚物种的热应激耐受性的贡献。