Introduction: Direct smearing preparation (conventional preparation [CP]) has been widely used for endometrial cytology in Japan. In CP, sampling and screening errors are problematic. In liquid-based cytology preparation (LBC), the problems of CP can be solved. But there is a problem that cytological findings of LBC are different from those of CP. The purpose of this study was to evaluate the differences of morphological findings of endometrial cytology between LBC and CP, and the usefulness of the endometrial LBC to differentiate endometrioid carcinoma grade 1 (G1) from grade 3 (G3). Methods: Thirteen cases of endometrioid carcinoma G1, and 5 cases of G3 collected by the Softcyte device and prepared by LBC and CP (split specimen) were used. We focused on the following items: (1) the number of clusters per cm², (2) the number of layers of clusters, (3) area of clusters, (4) perimeter of clusters, (5) roundness of clusters, (6) complexity of clusters, (7) area of nucleus, (8) perimeter of nucleus, (9) roundness of nucleus, (10) complexity of nucleus, (11) area of nucleolus, and (12) nucleolus-nucleus ratio (N/N). Results: Compared with CP, the number of clusters and layers of the clusters in LBC were significantly larger in G1. The area and perimeters of the clusters and the nucleus were significant smaller, and the N/N ratio was greater in LBC than that in CP in both G1 and G3. Regarding morphological differences between G1 and G3 in LBC and CP, the number of layers was significantly larger in G1 than in G3 in LBC and CP. The area of the clusters in LBC was significantly larger in G1 than in G3. The area and perimeters of the nucleus in CP and the area of the nucleolus and N/N ratio in LBC and CP were significantly smaller in G1 than in G3. Conclusion: In the endometrial cytology, it became clear that the cell image was different between LBC and CP and between G1 and G3. By microscopic examination understanding the characteristics of the cell image in LBC, endometrial LBC could be useful to diagnose endometrial carcinoma.
Acta Cytologica

中文翻译：

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子宫内膜子宫内膜样癌1级和3级液基细胞学与常规制剂之间的形态学差异以及每种方法的等级差异

简介：直接涂片制剂（常规制剂[CP]）在日本已广泛用于子宫内膜细胞学检查。在CP中，采样和筛选错误是有问题的。在基于液体的细胞学制剂（LBC）中，可以解决CP的问题。但是存在一个问题，即LBC的细胞学发现不同于CP的细胞学发现。本研究的目的是评估LBC和CP之间子宫内膜细胞学的形态学发现的差异，以及子宫内膜LBC区分1级（G1）和3级（G3）子宫内膜样癌的有用性。方法：使用Softcyte装置收集并由LBC和CP（分裂标本）制备的子宫内膜样癌G1 13例和G3 5例。我们关注以下项目：（1）每cm ²的簇数，（2）簇的层数，（3）簇的面积，（4）簇的周长，（5）簇的圆度，（ 6）簇的复杂性，（7）核的面积，（8）核的周长，（9）核的圆度，（10）核的复杂性，（11）核仁的面积，以及（12）核仁与核的比率（ N / N）。结果：与CP相比，G1中LBC中的簇数和簇的层数显着增加。在G1和G3中，LBC的簇和核的面积和周长都明显较小，N / N比大于CP。关于LBC和CP中G1和G3之间的形态差异，L1和CP中G1的层数明显大于G3。L1中LBC的簇的面积显着大于G3中的。在G1中，CP中核的面积和周长以及LBC和CP中核仁的面积和N / N比显着小于G3。结论：在子宫内膜细胞学中，很明显，LBC和CP以及G1和G3之间的细胞图像是不同的。通过显微镜检查了解LBC中细胞图像的特征，子宫内膜LBC可用于诊断子宫内膜癌。
细胞学学报