Searching for the associations between the gene polymorphism and the reproductive traits is essential in defining the genetic native breed specificity, which distinguishes them from the other breeds. The aim of our study was to determine the associations between mutations in the PRL, PRLR, PTGS2, FUT1 genes and sexual and periparturient activity in native Pulawska gilts. The analysis included 72 animals which gave birth to the first litter. Evaluation of the productive value of gilts accounted for indicators of sexual and periparturient activity as well as reproductive traits. The biological material for molecular analyses was obtained from the hair roots of the gilts. The genotype was verified by PCR RFLP analysis. The primers and PCR conditions were determined on the basis of available literature data. Statistically significant differences (P≤0.05) were found at the PRL locus: gilts of AA genotypes (Ins/Ins) at the PRL locus were characterised by longest farrowing duration compared to gilts of AB genotype (P≤0.05). The analysis of PRLR gene showed that gilts of TT genotype revealed a tendency for later occurrence of estrus signs (first and second estrus) and for the markedly longest farrowings (P≤0.05). With regard to PTGS2 and FUT1 loci, no significant differences were found in the parameters of sexual and periparturient activity of the gilts. However, gilts of FUT1 GG genotype gave birth to and reared the largest first litters (P≤0.05). The results of the studies expand the knowledge about the genetic structure and productivity of Pulawska gilts.

中文翻译：

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与选定的基因多态性相关联的Pulawska母猪发情行为和生殖性状

寻找基因多态性与生殖性状之间的联系对于定义遗传本地品种的特异性至关重要，这可以将它们与其他品种区分开来。我们研究的目的是确定PRL，PRLR，PTGS2，FUT1中突变之间的联系。本地Pulawska后备母猪的基因以及性和围产期活动。分析包括72头第一胎的动物。对小母猪的生产价值进行评估是性活动和围产期活动以及生殖性状的指标。从后备母猪的发根中获得了用于分子分析的生物材料。通过PCR RFLP分析验证基因型。引物和PCR条件是根据现有文献数据确定的。统计学显著差异（P≤0.05）被发现在PRL基因座：的小母猪AA基因型（宏/ INS在）PRL轨迹进行表征最长持续时间产仔相比的小母猪AB基因型（P≤0.05）。PRLR基因的分析表明，TT基因型的小母猪显示出出现发情迹象（第一和第二发情）的后期趋势和明显最长的产仔的趋势（P≤0.05）。关于PTGS2和FUT1基因座，后备母猪的性活动和围产期活动的参数没有发现显着差异。然而，FUT1 GG基因型的后备母猪产下并饲养了最大的第一胎（P≤0.05）。研究结果扩大了对Pulawska后备母猪遗传结构和生产力的认识。