ABSTRACT

The safety of transgenic Bt rice containing bacteria-derived mCry1Ac gene from Bacillus thuringiensis (Bt) was assessed by conducting field trials at two locations for two consecutive years in South Korea, using the near-isogenic line comparator rice cultivar (‘Ilmi’, non-Bt rice) and four commercial cultivars as references. Compositional analyses included measurement of proximates, minerals, amino acids, fatty acids, vitamins, and antinutrients. Significant differences between Bt rice and non-Bt rice were detected; however, all differences were within the reference range. The statistical analyses, including analysis of % variability, analysis of similarities (ANOISM), similarity percentage (SIMPER) analysis, and permutational multivariate analysis of variance (PERMANOVA) were performed to study factors contributing to compositional variability. The multivariate analyses revealed that environmental factors more influenced rice components’ variability than by genetic factors. This approach was shown to be a powerful method to provide meaningful evaluations between Bt rice and its comparators. In this study, Bt rice was proved to be compositionally equivalent to conventional rice varieties through multiple statistical methods.

摘要

含有苏云金芽孢杆菌菌源mCry1Ac基因的转基因Bt水稻的安全性(Bt) 是通过在韩国连续两年在两个地点进行田间试验来评估的，使用近等基因系比较水稻品种（'Ilmi'，非 Bt 水稻）和四个商业品种作为参考。成分分析包括测量近似物、矿物质、氨基酸、脂肪酸、维生素和抗营养素。检测到 Bt 水稻和非 Bt 水稻之间存在显着差异；然而，所有差异都在参考范围内。进行了统计分析，包括百分比变异性分析、相似性分析 (ANOISM)、相似性百分比 (SIMPER) 分析和置换多变量方差分析 (PERMANOVA)，以研究导致成分变异性的因素。多变量分析表明，环境因素比遗传因素对水稻成分变异的影响更大。这种方法被证明是一种强大的方法，可以在 Bt 水稻与其比较者之间进行有意义的评估。在这项研究中，通过多种统计方法证明 Bt 水稻在成分上与常规水稻品种相当。