Embryonic vascular development is achieved through the complex arrays of differentiation, proliferation, migration and mutual interaction of different cell types, and visualization as well as purification of unique cell populations are fundamental in studying its detailed mechanisms using in vivo experimental models. We previously demonstrated that Tmem100 was a novel endothelial gene encoding a small transmembrane protein, and that Tmem100 null mice showed embryonic lethality due to severe impairment of vascular formation. In the present study, we generated an EGFP reporter mouse line using a 216 kb genomic region containing mouse Tmem100 gene. A novel line designated as Tmem100‐BAC‐EGFP mice precisely recapitulated the Tmem100 expression profile at the mid‐gestational stage, which was highly enriched in endothelial cells of large caliber arteries in mouse embryos. FACS experiments demonstrated that Tmem100‐BAC‐EGFP mice served to selectively purify a specific population of arterial endothelial cells, indicating their usefulness not only for the research concerning Tmem100 expression and function but also for comparative analysis of multiple endothelial cell subgroups in embryonic vascular development.

中文翻译：

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Tmem100-BAC-EGFP 小鼠选择性标记和纯化妊娠中期血管形成中大口径动脉的胚胎内皮细胞

胚胎血管发育是通过不同细胞类型的分化、增殖、迁移和相互作用的复杂阵列来实现的，独特细胞群的可视化以及纯化是使用体内实验模型研究其详细机制的基础。我们之前证明Tmem100是一种新的内皮基因，编码一种小的跨膜蛋白，并且由于血管形成的严重受损， Tmem100缺失小鼠表现出胚胎致死率。在本研究中，我们使用包含小鼠Tmem100基因的 216 kb 基因组区域生成了 EGFP 报告小鼠系。一种被命名为Tmem100 -BAC-EGFP 小鼠的新品系精确地概括了Tmem100在妊娠中期的表达谱，在小鼠胚胎大口径动脉的内皮细胞中高度富集。FACS 实验表明，Tmem100 -BAC-EGFP 小鼠可选择性地纯化特定的动脉内皮细胞群，表明它们不仅可用于有关Tmem100表达和功能的研究，还可用于胚胎血管发育中多个内皮细胞亚群的比较分析。