High concentration of sodium chloride could induce the viable and culturable states of Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis,Letters in Applied Microbiology

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High concentration of sodium chloride could induce the viable and culturable states of Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2021-03-02 , DOI: 10.1111/lam.13468
Hana Song ₁ , Sun‐Young Lee ₁

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In the present study, Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis were transferred into Luria–Bertani medium without NaCl (LBWS) and adjusted to various pHs (4, 5, 6 and 7) with lactic acid containing 0·75, 5, 10 and 30% NaCl, and stored at 25°C until the bacterial populations reached below detectable levels on tryptic soy agar (TSA). Although E. coli O157:H7 and S. Enteritidis did not grow on TSA when incubated in LBWS with 30% NaCl for 35 and 7 days, more than 60 and 70% of the bacterial cells were shown to be viable via fluorescent staining with SYTO9 and propidium iodide (PI), respectively, suggesting that a number of cells could be induced into the viable but nonculturable (VBNC) state. These bacteria that were induced into a VBNC state were transferred to a newly prepared tryptic soy broth (TSB) and then incubated at 37°C for several days. After more than 7 days, E. coli O157:H7 and S. Enteritidis regained their culturability. We, therefore, suggest that E. coli O157:H7 and S. Enteritidis entered the VBNC state under the adverse condition of higher salt concentrations and were revived when these conditions were reversed.

中文翻译：

高浓度的氯化钠可诱导大肠杆菌O157：H7和肠炎沙门氏菌肠炎沙门氏菌的存活和培养状态

在本研究中，将大肠杆菌O157：H7和肠炎沙门氏菌肠炎沙门氏菌转移到不含NaCl（LBWS）的Luria-Bertani培养基中，并用含0·75、5的乳酸调节至各种pH值（4、5、6和7）。，10％和30％的NaCl，并保存在25°C下，直到细菌种群在胰蛋白酶大豆琼脂（TSA）上检测不到的水平以下为止。虽然ê。 大肠杆菌O157：H7和S。当在含有30％NaCl的LBWS中孵育35天和7天时，肠炎杆菌不会在TSA上生长，通过分别用SYTO9和碘化丙锭（PI）进行荧光染色显示，超过60％和70％的细菌细胞是可行的，这表明可以诱导许多细胞进入存活但不可培养的状态（VBNC）。将这些被诱导为VBNC状态的细菌转移到新制备的胰蛋白酶大豆肉汤（TSB）中，然后在37°C下孵育几天。超过7天后，E。 大肠杆菌O157：H7和小号。肠炎菌恢复了可培养性。因此，我们建议ē。 大肠杆菌O157：H7和S。肠炎在较高盐浓度的不利条件下进入VBNC状态，当这些条件逆转时，它们得以恢复。

更新日期：2021-05-11

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