We optimized ultrasound‐assisted alcohol‐based deep eutectic solvent dispersive liquid‐phase microextraction for separation and preconcentration of quercetin in wine and food samples by experimental design based on central composite design. Five different alcohol‐based deep eutectic solvents were prepared and tested for quercetin extraction. The effect of important parameters and matrix components were optimized. After optimization, the determination of quercetin was performed at 385 nm using spectrophotometry. Analytical data such as detection limit, working range and preconcentration factor were found as 6.1 μg/L, 20–850 μg/L, and 120, respectively. The selectivity of the optimized extraction conditions for quercetin was investigated in the presence of different matrices. The validation of the method was investigated by reproducibility, repeatability and recovery studies, as well as by comparing the analytical results obtained from real samples with the reference method. Lastly, the recommended procedure was successfully applied for the extraction and quantification of quercetin in wine and food samples.

中文翻译：

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响应面法优化超声辅助醇基深共熔溶剂分散液相微萃取分离和预浓缩葡萄酒和食品样品中的槲皮素

我们通过基于中心复合设计的实验设计，优化了超声辅助醇基深共熔溶剂分散液相微萃取，用于葡萄酒和食品样品中槲皮素的分离和预浓缩。制备了五种不同的醇基低共熔溶剂并测试了槲皮素的提取。优化了重要参数和基体成分的影响。优化后，槲皮素的测定在 385 nm 处使用s分光光度法。检测限、工作范围和预浓缩因子等分析数据分别为 6.1 μg/L、20–850 μg/L 和 120。在不同基质存在下研究了槲皮素的优化提取条件的选择性。通过再现性、重复性和回收率研究，以及通过将实际样品获得的分析结果与参考方法进行比较，对方法的验证进行了研究。最后，推荐的程序成功应用于葡萄酒和食品样品中槲皮素的提取和定量分析。