The use of plant growth-promoting fungi (PGPF) is a more environmentally friendly strategy than the use of fertilizers and pesticides in the field. These beneficial fungi have direct and indirect mechanisms that promote plant growth and protection. This study aimed to biochemically characterize the fungal Aspergillus niger 9-P isolated from native forage grass in the Pantanal of Nhecolândia region, concerning the production of plant growth-promoting (PGP) traits. The production of indole-3-acetic acid (IAA) was determined in relation to different physical-chemical parameters, phosphate solubilization, and production of siderophores by qualitative and quantitative methods. In addition, we investigated the production of ammonia, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and enzymes such as phosphatases, proteases, and other hydrolases (amylase, CMCase, pectinase, and xylanase) of agricultural interest. A pot experiment using common bean plants was carried out in a greenhouse for 30 days to evaluate the effect of the isolate on promoting plant growth. Briefly, the fungus showed the production of IAA (32.65 ± 1.26 µg mL^-1), siderophores (88% of siderophore units after 10 days), and high phosphorus solubilizing activity (1138.85 µg mL^-1). The isolate synthesized ACC deaminase, produced ammonia, and all other enzymes evaluated in this study. In addition, the results indicated that inoculation with the isolate provided higher values of height and fresh and dry mass of the aerial part and the root compared with the non-inoculated treatment of the common bean plant. The results of this study suggest the potential of the isolate A. niger 9-P for the formulation of biofertilizers aimed at promoting plant growth.

与在田间使用化肥和杀虫剂相比，使用植物促生长真菌（PGPF）是一种更环保的策略。这些有益真菌具有促进植物生长和保护的直接和间接机制。这项研究旨在生化表征真菌黑曲霉从Nhecolândia地区的潘塔纳尔湿地的天然饲料草中分离出的9-P，涉及植物促生长（PGP）特性的产生。通过定性和定量方法确定了与不同物理化学参数，磷酸盐增溶和铁载体的产生有关的吲哚-3-乙酸（IAA）的产生。此外，我们研究了氨，1-氨基环丙烷-1-羧酸（ACC）脱氨酶以及农业上感兴趣的酶的生产，例如磷酸酶，蛋白酶和其他水解酶（淀粉酶，CMCase，果胶酶和木聚糖酶）。使用普通豆类植物在温室中进行了30天的盆栽试验，以评估分离株对促进植物生长的影响。简而言之，真菌显示出IAA的产生（32.65±1.26 µg mL ^-1），铁载体（10天后占88％的铁载体）和高磷增溶活性（​​1138.85 µg mL ^-1）。该分离物合成了ACC脱氨酶，产生了氨气，并在本研究中评估了所有其他酶。另外，结果表明，与未接种的普通豆类植物相比，接种分离株可以提供更高的高度值，以及空中部分和根部的新鲜和干燥质量。这项研究的结果表明了分离株A的潜力。尼日尔9-P用于配制旨在促进植物生长的生物肥料。