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The influence of N-acetylglucosamine: Inducing Rhodosporidium paludigenum to enhance the inhibition of Penicillium expansum on pears
Postharvest Biology and Technology ( IF 7 ) Pub Date : 2021-03-02 , DOI: 10.1016/j.postharvbio.2021.111486
Yining Huang , Zhuoying Fan , Yiting Cai , Lifei Jin , Ting Yu

On the way to impel Rhodosporidium paludigenum to behave like a serviceable and beneficial biocontrol antagonist, it was found that adding different concentrations of N-acetylglucosamine (GlcNAc) during the culture can reduce the incidence of pears caused by Penicillium expansum. 0.1 % GlcNAc is an optimal concentration in the range of 0.001 %–2 %. R. paludigenum, cultivated and gathered from the medium with a final concentration of 0.1 % GlcNAc, declared the best impact on restraint on P. expansum on pears. This inhibitory effect is analogous to the consequence of spore germination experiments in vitro. The R. paludigenum gathered after GlcNAc induction, at the same time, displayed exceptional viability and vigour under different stress conditions, including sodium chloride (NaCl), calcofluor white (CFW), Congo red (CR) and sodium dodecyl sulfate (SDS). The enzyme activity and the relative gene expression levels of some antioxidant-related enzymes in R. paludigenum, including peroxisomal catalase (CAT), thioredoxin reductase (TrxR), glutathione peroxidase (GSH-PX), glutathione reductase (GR) and superoxide dismutase (SOD), were detected to be up-regulated in further testing. At the same time, the reactive oxygen species (ROS) remained at a low status.



中文翻译:

N-乙酰氨基葡糖的影响:诱导paludigenum Rhodosporidium paludigenum增强对梨的青霉菌的抑制

在促使苍白假单胞菌表现为有用且有益的生物防治拮抗剂的方式上,发现在培养过程中添加不同浓度的N-乙酰氨基葡萄糖(GlcNAc)可以减少由扩张青霉引起的梨的发生率。0.1%GlcNAc是0.001%–2%范围内的最佳浓度。从最终浓度为0.1%GlcNAc的培养基中培养和收集的paludigenum R.对抑制梨的P. expansum产生了最好的影响。这种抑制作用类似于体外孢子萌发实验的结果。该R. paludigenum同时,在GlcNAc诱导后收集到的NPC在不同的胁迫条件下表现出出色的生存力和活力,包括氯化钠(NaCl),钙氟白色(CFW),刚果红(CR)和十二烷基硫酸钠(SDS)。paludigenum中某些抗氧剂相关酶的酶活性和相对基因表达水平,包括过氧化物酶体过氧化氢酶(CAT),硫氧还蛋白还原酶(TrxR),谷胱甘肽过氧化物酶(GSH-PX),谷胱甘肽还原酶(GR)和超氧化物歧化酶( SOD)被发现在进一步测试中被上调。同时,活性氧(ROS)保持在较低的状态。

更新日期:2021-03-02
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